PMID- 1910735
OWN - NLM
STAT- MEDLINE
DCOM- 19911104
LR  - 20190912
IS  - 0829-8211 (Print)
IS  - 0829-8211 (Linking)
VI  - 69
IP  - 5-6
DP  - 1991 May-Jun
TI  - Analysis of RNA for transcripts for catalase and SP71 in rat hearts after in vivo 
      hyperthermia.
PG  - 375-82
AB  - Hyperthermic stress induces synthesis of the major inducible (heat) stress 
      protein (SP71) in all rat tissues. In addition, there is an increase in catalase 
      activity in hearts at 24 and 48 h after the induction of the heat shock response. 
      To more precisely define some of the molecular aspects of the induction of the 
      heat shock response in hearts, we examined mRNA levels for the catalase, SP71 and 
      HSP27. RNA was isolated from control hearts and at various time periods (0-24 h) 
      of recovery after brief hyperthermic treatment and was analyzed by Northern blot 
      analysis using as probes cDNA sequences for rat liver catalase, human HSP70 
      (inducible), and human HSP27. There was no detectable change in mRNA for catalase 
      after heat shock or during recovery. Hyperthermic stress has no apparent effect 
      on the regulation of transcription of mRNA coding for catalase, indicating that 
      the increase in catalase activity is either translationally or 
      post-translationally regulated. The human HSP70 cDNA did not hybridize to control 
      heart RNA, but did hybridize to SP71 transcripts at 0, 1.5, and 3 h post heat 
      shock. The mRNA level for SP71 peaked at 1.5 h, was reduced at 3 h, and became 
      almost undetectable at 6 h post heat shock. Similarly, the human HSP27 cDNA did 
      not hybridize to control heart RNA, but did hybridize to transcripts for HSP27 at 
      0, 1.5, 3, and up to 15 h post heat shock. Maximal signal for HSP27 was at 3 h 
      post heat shock and was sharply reduced at 6 h post heat shock.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Currie, R W
AU  - Currie RW
AD  - Department of Anatomy, Dalhousie University, Halifax, N.S., Canada.
FAU - Tanguay, R M
AU  - Tanguay RM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Catalase/analysis/biosynthesis/*genetics
MH  - Fever/*genetics/metabolism
MH  - Heat-Shock Proteins/biosynthesis/*genetics
MH  - Humans
MH  - Male
MH  - Myocardium/*metabolism
MH  - Nucleic Acid Hybridization
MH  - Plasmids/genetics
MH  - RNA, Messenger/*analysis/genetics/isolation & purification
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Transcription, Genetic
EDAT- 1991/05/01 00:00
MHDA- 1991/05/01 00:01
CRDT- 1991/05/01 00:00
PHST- 1991/05/01 00:00 [pubmed]
PHST- 1991/05/01 00:01 [medline]
PHST- 1991/05/01 00:00 [entrez]
AID - 10.1139/o91-057 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 1991 May-Jun;69(5-6):375-82. doi: 10.1139/o91-057.