PMID- 19107402
OWN - NLM
STAT- MEDLINE
DCOM- 20090331
LR  - 20211020
IS  - 1225-8873 (Print)
IS  - 1225-8873 (Linking)
VI  - 46
IP  - 6
DP  - 2008 Dec
TI  - Molecular characterization of ORFs 2 to 7 of Korean porcine reproductive and 
      respiratory syndrome virus (CA) and its protein expression by recombinant 
      baculoviruses.
PG  - 709-19
LID - 10.1007/s12275-008-0224-x [doi]
AB  - To determine the characteristics of the Korean porcine reproductive and 
      respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an 
      infected pig in 2006, we investigated the nucleotide sequence and expression of 
      the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the 
      structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as 
      those formed from VR-2332. Comparison of the CA with the other strains revealed 
      nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the 
      genetic relationships between other strains, phylogenetic analyses were 
      performed. The CA strain was closely related to the other North American genotype 
      strains but formed a distinct branch with high bootstrap support. Additionally, 
      expression levels of the PRRSV proteins in insect cells were strong or partially 
      weak. The results of this study have implications for both the taxonomy of PRRSV 
      and vaccine development.
FAU - Koo, Hyun Na
AU  - Koo HN
AD  - Department of Plant Medicine, College of Agriculture, Life and Environment 
      Sciences, Chungbuk National University, Cheongju, 361-763, Republic of Korea.
FAU - Oh, Jeong Mi
AU  - Oh JM
FAU - Lee, Jae Kyung
AU  - Lee JK
FAU - Choi, Jae Young
AU  - Choi JY
FAU - Lee, Kwang Sik
AU  - Lee KS
FAU - Roh, Jong Yul
AU  - Roh JY
FAU - Je, Yeon Ho
AU  - Je YH
FAU - Jin, Byung Rae
AU  - Jin BR
FAU - Yoo, Sung Sik
AU  - Yoo SS
FAU - Kim, Jae Su
AU  - Kim JS
FAU - Kim, Young In
AU  - Kim YI
FAU - Yoon, In Joong
AU  - Yoon IJ
FAU - Woo, Soo Dong
AU  - Woo SD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081224
PL  - Korea (South)
TA  - J Microbiol
JT  - Journal of microbiology (Seoul, Korea)
JID - 9703165
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Animals
MH  - Baculoviridae/genetics/*metabolism
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Korea
MH  - Molecular Sequence Data
MH  - Open Reading Frames/*genetics
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*classification/*genetics/isolation & purification/metabolism
MH  - *Recombination, Genetic
MH  - Sequence Alignment
MH  - Spodoptera/virology
MH  - Swine
MH  - Viral Structural Proteins/genetics/*metabolism
EDAT- 2008/12/25 09:00
MHDA- 2009/04/01 09:00
CRDT- 2008/12/25 09:00
PHST- 2008/09/11 00:00 [received]
PHST- 2008/09/30 00:00 [accepted]
PHST- 2008/12/25 09:00 [entrez]
PHST- 2008/12/25 09:00 [pubmed]
PHST- 2009/04/01 09:00 [medline]
AID - 10.1007/s12275-008-0224-x [doi]
PST - ppublish
SO  - J Microbiol. 2008 Dec;46(6):709-19. doi: 10.1007/s12275-008-0224-x. Epub 2008 Dec 
      24.