PMID- 19107978
OWN - NLM
STAT- MEDLINE
DCOM- 20090310
LR  - 20081229
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Linking)
VI  - 81
IP  - 2
DP  - 2009 Feb
TI  - Prevalence of HHV-6 integrated chromosomally among children treated for acute 
      lymphoblastic or myeloid leukemia in the Czech Republic.
PG  - 258-63
LID - 10.1002/jmv.21371 [doi]
AB  - Chromosomal integration of human herpesvirus 6 (HHV-6) is a novel situation found 
      in a small percentage of individuals. While active HHV-6 infection is treatable 
      using antivirals, the abnormally high level of HHV-6 DNA found in chromosomal 
      integration of HHV-6 (CI-HHV-6) is not affected by such drugs. Stored DNA samples 
      taken originally for detection of fusion genes and minimal residual disease from 
      339 pediatric patients treated for leukemia in the Czech Republic between the 
      years 1995-2007 were tested retrospectively. Using real-time quantitative PCR 
      technology, the quantity of HHV-6 DNA detected was normalized to 100,000 human 
      genome equivalents as assessed by quantitation of the albumin gene. HHV-6 DNA was 
      detected in 107 samples from 91 patients (26.8%). In the majority of samples (99) 
      only a minute level of normalized viral copies (NVCs) (median 1.84 NVCs) was 
      detected. A high viral load of approximately 100,000 NVCs was detected in 5 
      patients (1.5%; median 140,150 NVCs), in all of whom were confirmed subsequently 
      CI-HHV-6 by a detection of HHV-6 DNA in hair follicles or in the nails. In all 
      but one patient with HHV-6 variant B, variant A of the virus was detected. None 
      of the patients with CI-HHV-6 had complications attributable to HHV-6 infection. 
      The prevalence of CI-HHV-6 in childhood leukemia does not differ from that 
      published for other patients or healthy populations. Where high levels of HHV-6 
      DNA are present, CI-HHV-6 should be confirmed as soon as possible so that 
      potentially toxic but ineffective antiviral treatment can be stopped.
CI  - (c) 2008 Wiley-Liss, Inc.
FAU - Hubacek, Petr
AU  - Hubacek P
AD  - Department of Paediatric Haematology and Oncology, Motol University Hospital, 
      Prague, Czech Republic. petr.hubacek@lfmotol.cuni.cz
FAU - Muzikova, Katerina
AU  - Muzikova K
FAU - Hrdlickova, Alena
AU  - Hrdlickova A
FAU - Cinek, Ondrej
AU  - Cinek O
FAU - Hyncicova, Katerina
AU  - Hyncicova K
FAU - Hrstkova, Hana
AU  - Hrstkova H
FAU - Sedlacek, Petr
AU  - Sedlacek P
FAU - Stary, Jan
AU  - Stary J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Blood/virology
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes/*virology
MH  - Czech Republic/epidemiology
MH  - DNA, Viral/analysis/genetics
MH  - Female
MH  - Herpesviridae Infections/diagnosis/*epidemiology/virology
MH  - Herpesvirus 6, Human/*genetics/*isolation & purification
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Leukemia, Myeloid/*virology
MH  - Male
MH  - Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/*virology
MH  - Prevalence
MH  - Retrospective Studies
MH  - Virus Integration/*genetics
EDAT- 2008/12/25 09:00
MHDA- 2009/03/11 09:00
CRDT- 2008/12/25 09:00
PHST- 2008/12/25 09:00 [entrez]
PHST- 2008/12/25 09:00 [pubmed]
PHST- 2009/03/11 09:00 [medline]
AID - 10.1002/jmv.21371 [doi]
PST - ppublish
SO  - J Med Virol. 2009 Feb;81(2):258-63. doi: 10.1002/jmv.21371.