PMID- 19108843
OWN - NLM
STAT- MEDLINE
DCOM- 20090715
LR  - 20131121
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1216
IP  - 19
DP  - 2009 May 8
TI  - Purification of high-throughput organic synthesis libraries by counter-current 
      chromatography.
PG  - 4154-60
LID - 10.1016/j.chroma.2008.11.092 [doi]
AB  - Experiments were performed to evaluate whether counter-current chromatography 
      (CCC) could function as an alternative purification method to reversed-phase 
      high-performance liquid chromatography (RP-HPLC) and normal-phase supercritical 
      fluid chromatography (SFC). RP-HPLC and SFC are the routine methods currently 
      used in our high-throughput purification (HTP) facility for the purification of 
      high-throughput organic synthesis (HTOS) libraries and medicinal chemistry 
      reaction mixtures. Pre-equilibration of the solvent mixture layers was not 
      mandatory for effective chromatography when hexanes-ethyl acetate-methanol-water 
      (HEMW) solvent mixtures were used. Key to the use of CCC for high-throughput 
      applications is the ability to effectively select a solvent system appropriate to 
      each library member. Pilot-scale CCC elution time was used to estimate a starting 
      solvent ratio and RP-HPLC retention time was then used to adjust solvent ratios 
      within a particular library. It was also found that dimethyl sulfoxide (DMSO) and 
      DMSO-methanol were suitable as sample injection solvents when using the HEMW 
      solvent systems.
FAU - Wagenaar, Frank L
AU  - Wagenaar FL
AD  - Abbott Laboratories, Abbott Park, IL 60064, USA. frank.wagenaar@abbott.com
FAU - Hochlowski, Jill E
AU  - Hochlowski JE
FAU - Pan, Jeffrey Y
AU  - Pan JY
FAU - Tu, Noah P
AU  - Tu NP
FAU - Searle, Philip A
AU  - Searle PA
LA  - eng
PT  - Journal Article
DEP - 20081206
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Organic Chemicals)
RN  - 0 (Small Molecule Libraries)
RN  - 0 (Solvents)
RN  - 90Y4QC304K (Ketoprofen)
RN  - WK2XYI10QM (Ibuprofen)
RN  - Y4S76JWI15 (Methanol)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Acylation
MH  - Chromatography, High Pressure Liquid
MH  - Countercurrent Distribution/*methods
MH  - Dimethyl Sulfoxide/chemistry
MH  - Equipment Design
MH  - Ibuprofen/isolation & purification
MH  - Ketoprofen/isolation & purification
MH  - Methanol/chemistry
MH  - Organic Chemicals/*chemistry
MH  - *Small Molecule Libraries
MH  - Solubility
MH  - Solvents/chemistry
EDAT- 2008/12/26 09:00
MHDA- 2009/07/16 09:00
CRDT- 2008/12/26 09:00
PHST- 2008/09/30 00:00 [received]
PHST- 2008/11/20 00:00 [revised]
PHST- 2008/11/25 00:00 [accepted]
PHST- 2008/12/26 09:00 [entrez]
PHST- 2008/12/26 09:00 [pubmed]
PHST- 2009/07/16 09:00 [medline]
AID - S0021-9673(08)02113-4 [pii]
AID - 10.1016/j.chroma.2008.11.092 [doi]
PST - ppublish
SO  - J Chromatogr A. 2009 May 8;1216(19):4154-60. doi: 10.1016/j.chroma.2008.11.092. 
      Epub 2008 Dec 6.