PMID- 19108975
OWN - NLM
STAT- MEDLINE
DCOM- 20090318
LR  - 20131121
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 32
IP  - 1
DP  - 2009 Feb
TI  - Effect of seed sludge and operation conditions on performance and archaeal 
      community structure of low-temperature anaerobic solvent-degrading bioreactors.
PG  - 65-79
LID - 10.1016/j.syapm.2008.10.003 [doi]
AB  - Two laboratory-scale expanded granular sludge bed (EGSB) anaerobic bioreactors 
      (R1 and R2) were inoculated with biomass from different mesophilic (37 degrees C) 
      treatment plants, and used for the treatment of an organic solvent-based 
      wastewater at 9-14 degrees C at applied organic loading rates (OLRs) of 1.2-3.6kg 
      chemical oxygen demand (COD)m(-3)d(-1). Replicated treatment performance was 
      observed at 10-14 degrees C, which suggested the feasibility of the process at 
      pilot-scale. Stable and efficient COD removal, along with high methane 
      productivity, was demonstrated at 9 degrees C at an applied OLR of 
      2.4kgCODm(-3)d(-1). Clonal libraries and fluorescence in situ hybridization 
      (FISH) indicated that the seed sludges were dominated (>60%) by acetoclastic 
      Methanosaeta-like organisms. Specific methanogenic activity (SMA) profiles 
      indicated shifts in the physiological profiles of R1 and R2 biomass, including 
      the development of psychrotolerant methanogenic activity. Acetoclastic 
      methanogenesis represented the primary route of methane production in R1 and R2, 
      which is in contrast with several previous reports from low-temperature 
      bioreactor trials. A reduction in the abundance of Methanosaeta-like clones (R2), 
      along with the detection of hydrogenotrophic methanogenic species, coincided with 
      altered granule (sludge) morphology and the development of hydrogenotrophic SMA 
      after prolonged operation at 9 degrees C.
FAU - Enright, Anne-Marie
AU  - Enright AM
AD  - Microbial Ecology Laboratory, Department of Microbiology and Environmental Change 
      Institute (ECI), National University of Ireland, Galway (NUI, Galway), University 
      Road, Galway, Ireland.
FAU - McGrath, Vincent
AU  - McGrath V
FAU - Gill, Darryl
AU  - Gill D
FAU - Collins, Gavin
AU  - Collins G
FAU - O'Flaherty, Vincent
AU  - O'Flaherty V
LA  - eng
SI  - GENBANK/DQ399813
SI  - GENBANK/DQ399814
SI  - GENBANK/DQ399815
SI  - GENBANK/DQ399816
SI  - GENBANK/DQ399817
SI  - GENBANK/DQ462726
SI  - GENBANK/DQ462727
SI  - GENBANK/DQ462728
SI  - GENBANK/DQ462729
SI  - GENBANK/DQ462730
SI  - GENBANK/DQ462731
SI  - GENBANK/DQ679903
PT  - Journal Article
DEP - 20081223
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (DNA, Archaeal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 0 (Solvents)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Anaerobiosis
MH  - Archaea/classification/genetics/*growth & development/metabolism
MH  - *Bioreactors
MH  - Biotechnology
MH  - DNA, Archaeal/analysis
MH  - Drug Industry
MH  - *Ecosystem
MH  - In Situ Hybridization, Fluorescence
MH  - Methane/biosynthesis
MH  - Methanosarcinales/classification/genetics/growth & development/metabolism
MH  - Molecular Sequence Data
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Solvents/chemistry/*metabolism
MH  - *Temperature
MH  - Waste Disposal, Fluid/methods
EDAT- 2008/12/26 09:00
MHDA- 2009/03/19 09:00
CRDT- 2008/12/26 09:00
PHST- 2008/07/07 00:00 [received]
PHST- 2008/10/25 00:00 [revised]
PHST- 2008/10/31 00:00 [accepted]
PHST- 2008/12/26 09:00 [entrez]
PHST- 2008/12/26 09:00 [pubmed]
PHST- 2009/03/19 09:00 [medline]
AID - S0723-2020(08)00126-4 [pii]
AID - 10.1016/j.syapm.2008.10.003 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2009 Feb;32(1):65-79. doi: 10.1016/j.syapm.2008.10.003. Epub 
      2008 Dec 23.