PMID- 19109449
OWN - NLM
STAT- MEDLINE
DCOM- 20090309
LR  - 20211020
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 16
IP  - 2
DP  - 2009 Feb
TI  - Streptococcus suis bacterin and subunit vaccine immunogenicities and protective 
      efficacies against serotypes 2 and 9.
PG  - 200-8
LID - 10.1128/CVI.00371-08 [doi]
AB  - Streptococcus suis causes numerous diseases in pigs, most importantly, 
      meningitis, arthritis, septicemia, and bronchopneumonia. One of the major 
      problems in modern swine production is the lack of a vaccine protecting against 
      more than one S. suis serotype. The objective of this study was to determine the 
      protective efficacy of a serotype 2 murein-associated protein (MAP) fraction 
      subunit vaccine in comparison to that of a bacterin against experimental 
      challenge with serotype 2 (containing muramidase-released protein [MRP], 
      extracellular factor, and suilysin [SLY]) and serotype 9 (containing MRP variant 
      MRP* and SLY) strains. MAP was shown to include different surface-associated 
      proteins, such as the MRP and surface antigen one (SAO) expressed by both 
      pathotypes used for challenge. The results of this study demonstrated that the 
      serotype 2 bacterin induced protective immunity against homologous challenge. In 
      contrast, the protective efficacy of the MAP subunit vaccine was low, though MAP 
      immunization resulted in high serum immunoglobulin G2 titers against MRP and SAO. 
      Importantly, immunization with bacterin but not with MAP induced opsonizing 
      antibody titers against the serotype 2 strain, and these antibody titers were 
      found to correlate with protection. However, after absorption with a 
      nonencapsulated isogenic mutant, the sera from bacterin-immunized piglets failed 
      to facilitate neutrophil killing, indicating that antibodies directed against 
      capsule may not have been essential for opsonophagocytosis. Furthermore, 
      induction of opsonizing antibodies against serotype 9 was not detectable in the 
      group receiving bacterin or in the group receiving the MAP vaccine. In agreement, 
      protection against the heterologous serotype 9 strain was low in both groups. 
      Thus, identification of an antigen protecting against these two important S. suis 
      pathotypes remains an important goal of future studies.
FAU - Baums, Christoph Georg
AU  - Baums CG
AD  - Institut fur Mikrobiologie, Zentrum fur Infektionsmedizin, Stiftung Tierarztliche 
      Hochschule Hannover, D-30173 Hannover, Germany. christoph.baums@gmx.de
FAU - Kock, Christoph
AU  - Kock C
FAU - Beineke, Andreas
AU  - Beineke A
FAU - Bennecke, Katharina
AU  - Bennecke K
FAU - Goethe, Ralph
AU  - Goethe R
FAU - Schroder, Charlotte
AU  - Schroder C
FAU - Waldmann, Karl-Heinz
AU  - Waldmann KH
FAU - Valentin-Weigand, Peter
AU  - Valentin-Weigand P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081224
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Opsonin Proteins)
RN  - 0 (Vaccines, Subunit)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Bacterial Capsules/immunology
MH  - Bacterial Proteins/*immunology
MH  - Bacterial Vaccines/*immunology
MH  - Opsonin Proteins/immunology
MH  - Phagocytosis/immunology
MH  - Streptococcal Infections/prevention & control/*veterinary
MH  - Streptococcus suis/*immunology
MH  - Swine
MH  - Swine Diseases/immunology/*prevention & control
MH  - Vaccines, Subunit/immunology
PMC - PMC2643536
EDAT- 2008/12/26 09:00
MHDA- 2009/03/10 09:00
PMCR- 2009/08/01
CRDT- 2008/12/26 09:00
PHST- 2008/12/26 09:00 [entrez]
PHST- 2008/12/26 09:00 [pubmed]
PHST- 2009/03/10 09:00 [medline]
PHST- 2009/08/01 00:00 [pmc-release]
AID - CVI.00371-08 [pii]
AID - 0371-08 [pii]
AID - 10.1128/CVI.00371-08 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2009 Feb;16(2):200-8. doi: 10.1128/CVI.00371-08. Epub 2008 
      Dec 24.