PMID- 19109691
OWN - NLM
STAT- MEDLINE
DCOM- 20090821
LR  - 20211020
IS  - 1433-4909 (Electronic)
IS  - 1431-0651 (Linking)
VI  - 13
IP  - 2
DP  - 2009 Mar
TI  - Differential microbial communities in hot spring mats from Western Thailand.
PG  - 321-31
LID - 10.1007/s00792-008-0219-x [doi]
AB  - The microbial communities of freshwater hot spring mats from Boekleung (Western 
      Thailand) were studied. Temperatures ranged from over 50 up to 57 degrees C. 
      Green-, red-, and yellow colored mat layers were analyzed. In order to detect the 
      major components of the microbial communities constituting the mat as well as the 
      microorganisms showing significant metabolic activity, samples were analyzed 
      using DNA- and RNA-based molecular techniques, respectively. Microbial community 
      fingerprints, performed by denaturing gradient gel electrophoresis (DGGE), 
      revealed clear differences among mat layers. Thermophilic phototrophic 
      microorganisms, Cyanobacteria and Chloroflexi, constituted the major groups in 
      these communities (on average 65 and 51% from DNA and RNA analyses, 
      respectively). Other bacteria detected in the mat were Bacteroidetes, members of 
      the Candidate Division OP10, Actinobacteria, and Planctomycetes. Differently 
      colored mat layers showed characteristic bacterial communities and the major 
      components of the metabolically active fraction of these communities have been 
      identified.
FAU - Portillo, M C
AU  - Portillo MC
AD  - Institute of Natural Resources and Agrobiology, IRNAS-CSIC, Avda. Reina Mercedes 
      10, 41012, Sevilla, Spain.
FAU - Sririn, V
AU  - Sririn V
FAU - Kanoksilapatham, W
AU  - Kanoksilapatham W
FAU - Gonzalez, J M
AU  - Gonzalez JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081225
PL  - Germany
TA  - Extremophiles
JT  - Extremophiles : life under extreme conditions
JID - 9706854
SB  - IM
MH  - Actinobacteria/*physiology
MH  - Bacteria/genetics
MH  - Bacteroidetes/*physiology
MH  - Biodiversity
MH  - Chloroflexi/*physiology
MH  - Cyanobacteria/*physiology
MH  - Ecosystem
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Fresh Water/microbiology
MH  - Hot Springs/microbiology
MH  - Phylogeny
MH  - Sequence Analysis, DNA
MH  - Thailand
MH  - Water Microbiology
EDAT- 2008/12/26 09:00
MHDA- 2009/08/22 09:00
CRDT- 2008/12/26 09:00
PHST- 2008/07/24 00:00 [received]
PHST- 2008/12/03 00:00 [accepted]
PHST- 2008/12/26 09:00 [entrez]
PHST- 2008/12/26 09:00 [pubmed]
PHST- 2009/08/22 09:00 [medline]
AID - 10.1007/s00792-008-0219-x [doi]
PST - ppublish
SO  - Extremophiles. 2009 Mar;13(2):321-31. doi: 10.1007/s00792-008-0219-x. Epub 2008 
      Dec 25.