PMID- 19109814
OWN - NLM
STAT- MEDLINE
DCOM- 20090318
LR  - 20191210
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 115
IP  - 2
DP  - 2009 Jan 15
TI  - Bacterial artificial chromosome array-based comparative genomic hybridization 
      using paired formalin-fixed, paraffin-embedded and fresh frozen tissue specimens 
      in multiple myeloma.
PG  - 345-54
LID - 10.1002/cncr.24021 [doi]
AB  - BACKGROUND: Multiple myeloma (MM) is a neoplasm of malignant plasma cells that 
      often harbors many chromosomal aberrations. Currently, fresh frozen tissues (FT) 
      are considered the most reliable for molecular genetic analysis; however, 
      formalin-fixed, paraffin-embedded (FFPE) tissues are easily retrievable. Compared 
      with conventional cytogenetics, bacterial artificial chromosome (BAC) 
      array-comparative genomic hybridization (CGH) allows more sensitive detection of 
      chromosomal abnormalities. METHODS: The authors analyzed 7 paired FT and FFPE 
      samples of bone marrow aspirate materials obtained from patients with MM in 
      parallel to determine the efficacy of BAC array-CGH using FFPE. RESULTS: 
      Thirty-four aberrations were identified, including 29 that were observed in both 
      sample types, yielding 85% concordance. Nonrandom anomalies, including gains on 
      7q, 9q, 15q, and 19p and losses on 8p and 13q, were observed in paired samples 
      from at least 2 patients. To verify these results, fluorescence in situ 
      hybridization (FISH) was performed using probes specific for 7q and 15q, and 
      gains were observed in the 4 samples that were examined. Furthermore, 1 of 3 
      samples from patients who had monoclonal gammopathy of undetermined significance 
      that were tested also carried gain on 7q, suggesting that this aberration may be 
      an early transforming event. CONCLUSIONS: The current results indicated that BAC 
      array-CGH can be effective using FFPE samples and is a sensitive method for the 
      identification of nonrandom chromosomal aberrations in MM.
CI  - Copyright (c) 2009 American Cancer Society.
FAU - Lennon, Patrick A
AU  - Lennon PA
AD  - School of Health Sciences, Department of Hematopathology, the University of Texas 
      M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
FAU - Zhuang, Yi
AU  - Zhuang Y
FAU - Pierson, Diane
AU  - Pierson D
FAU - Zhang, Xiang
AU  - Zhang X
FAU - Williams, Chris
AU  - Williams C
FAU - Perez, Cintia
AU  - Perez C
FAU - Lin, Pei
AU  - Lin P
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Aged
MH  - Bone Marrow Cells
MH  - Chromosome Aberrations
MH  - *Chromosomes, Artificial, Bacterial
MH  - Comparative Genomic Hybridization/*methods
MH  - Female
MH  - Frozen Sections
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
MH  - *Paraffin Embedding/methods
MH  - Tissue Array Analysis
EDAT- 2008/12/26 09:00
MHDA- 2009/03/19 09:00
CRDT- 2008/12/26 09:00
PHST- 2008/12/26 09:00 [entrez]
PHST- 2008/12/26 09:00 [pubmed]
PHST- 2009/03/19 09:00 [medline]
AID - 10.1002/cncr.24021 [doi]
PST - ppublish
SO  - Cancer. 2009 Jan 15;115(2):345-54. doi: 10.1002/cncr.24021.