PMID- 19109895
OWN - NLM
STAT- MEDLINE
DCOM- 20090112
LR  - 20240109
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 135
IP  - 7
DP  - 2008 Dec 26
TI  - Translation initiation on mammalian mRNAs with structured 5'UTRs requires 
      DExH-box protein DHX29.
PG  - 1237-50
LID - 10.1016/j.cell.2008.10.037 [doi]
AB  - Eukaryotic protein synthesis begins with assembly of 48S initiation complexes at 
      the initiation codon of mRNA, which requires at least seven initiation factors 
      (eIFs). First, 43S preinitiation complexes comprising 40S ribosomal subunits, 
      eIFs 3, 2, 1, and 1A, and tRNA(Met)(i) attach to the 5'-proximal region of mRNA 
      and then scan along the 5' untranslated region (5'UTR) to the initiation codon. 
      Attachment of 43S complexes is mediated by three other eIFs, 4F, 4A, and 4B, 
      which cooperatively unwind the cap-proximal region of mRNA and later also assist 
      43S complexes during scanning. We now report that these seven eIFs are not 
      sufficient for efficient 48S complex formation on mRNAs with highly structured 
      5'UTRs, and that this process requires the DExH-box protein DHX29. DHX29 binds 
      40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. NTP hydrolysis by DHX29 is 
      strongly stimulated by 43S complexes and is required for DHX29's activity in 
      promoting 48S complex formation.
FAU - Pisareva, Vera P
AU  - Pisareva VP
AD  - Department of Microbiology and Immunology, SUNY Downstate Medical Center, 450 
      Clarkson Avenue, Brooklyn, NY 11203, USA.
FAU - Pisarev, Andrey V
AU  - Pisarev AV
FAU - Komar, Anton A
AU  - Komar AA
FAU - Hellen, Christopher U T
AU  - Hellen CU
FAU - Pestova, Tatyana V
AU  - Pestova TV
LA  - eng
GR  - R01 GM059660/GM/NIGMS NIH HHS/United States
GR  - AI051340/AI/NIAID NIH HHS/United States
GR  - R01 AI051340/AI/NIAID NIH HHS/United States
GR  - GM059660/GM/NIGMS NIH HHS/United States
GR  - R01 AI051340-06A1/AI/NIAID NIH HHS/United States
GR  - R01 GM059660-08/GM/NIGMS NIH HHS/United States
GR  - R56 AI051340/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.7.- (DHX29 protein, human)
RN  - EC 3.6.4.13 (RNA Helicases)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Animals
MH  - HeLa Cells
MH  - Humans
MH  - *Protein Biosynthesis
MH  - RNA Helicases/*metabolism
MH  - RNA, Messenger/*genetics
MH  - Rabbits
MH  - Reticulocytes/metabolism
PMC - PMC2948571
MID - NIHMS85705
EDAT- 2008/12/27 09:00
MHDA- 2009/01/13 09:00
PMCR- 2010/10/01
CRDT- 2008/12/27 09:00
PHST- 2008/05/01 00:00 [received]
PHST- 2008/08/01 00:00 [revised]
PHST- 2008/10/21 00:00 [accepted]
PHST- 2008/12/27 09:00 [entrez]
PHST- 2008/12/27 09:00 [pubmed]
PHST- 2009/01/13 09:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - S0092-8674(08)01374-3 [pii]
AID - 10.1016/j.cell.2008.10.037 [doi]
PST - ppublish
SO  - Cell. 2008 Dec 26;135(7):1237-50. doi: 10.1016/j.cell.2008.10.037.