PMID- 19109933
OWN - NLM
STAT- MEDLINE
DCOM- 20090318
LR  - 20090203
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1255
DP  - 2009 Feb 19
TI  - Exposure to cerebrospinal fluid of sporadic amyotrophic lateral sclerosis 
      patients alters Nav1.6 and Kv1.6 channel expression in rat spinal motor neurons.
PG  - 170-9
LID - 10.1016/j.brainres.2008.11.099 [doi]
AB  - Cerebro Spinal Fluid (CSF) from patients with ALS has been documented to have a 
      toxic effect on motor neurons both in vivo and in vitro. Here we show that the 
      CSF from Amyotrophic Lateral Sclerosis (ALS) patients (ALS-CSF) has the potential 
      to perturb ion channel expression, specifically the Na(v)1.6, and K(v)1.6 
      channels in newborn rat spinal motor neurons both in vivo and in vitro. ALS-CSF 
      and CSF from nonALS patients (nonALS-CSF) were intrathecally injected into 
      3-day-old rat pups at the rate of 1 microl/2.5 min using a microinjector. In 
      addition, embryonic rat spinal cord cultures were also exposed to 10% ALS or 
      nonALS-CSF on the 9th day in vitro (9DIV) in serum free DMEM medium. After 48 h 
      of CSF exposure, the cultures and the spinal cord sections were processed for 
      immunostaining of the above mentioned ion channels. We observed a decrease in the 
      expression of Na(v)1.6 and K(v)1.6 channels in motor neurons in ALS-CSF treated 
      group, and the presence of trophic factors like Brain Derived Neurotrophic Factor 
      (BDNF) and Ciliary Neurotrophic Factor CNTF partially reversed the effects 
      produced by ALS-CSF. Altered expression of these voltage-gated channels may 
      interfere with the electrical activity of motor neurons, and thereby lead to the 
      degeneration of neurons.
FAU - Gunasekaran, R
AU  - Gunasekaran R
AD  - Department of Neurophysiology, National Institute of Mental Health and Neuro 
      Sciences, Post Box no: 2900, Hosur Road, Bangalore-560 029, India.
FAU - Narayani, R Sankara
AU  - Narayani RS
FAU - Vijayalakshmi, K
AU  - Vijayalakshmi K
FAU - Alladi, Phalguni Anand
AU  - Alladi PA
FAU - Shobha, K
AU  - Shobha K
FAU - Nalini, A
AU  - Nalini A
FAU - Sathyaprabha, T N
AU  - Sathyaprabha TN
FAU - Raju, T R
AU  - Raju TR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081211
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Biological Factors)
RN  - 0 (Kv1.6 Potassium Channel)
RN  - 0 (Sodium Channels)
SB  - IM
MH  - Amyotrophic Lateral Sclerosis/*metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Biological Factors/cerebrospinal fluid/*toxicity
MH  - Cells, Cultured
MH  - *Cerebrospinal Fluid
MH  - Gene Expression Regulation
MH  - Humans
MH  - Injections, Spinal
MH  - Kv1.6 Potassium Channel/*metabolism
MH  - Microinjections
MH  - Motor Neurons/*drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium Channels/*metabolism
MH  - Spinal Cord/cytology/drug effects/metabolism
EDAT- 2008/12/27 09:00
MHDA- 2009/03/19 09:00
CRDT- 2008/12/27 09:00
PHST- 2008/09/12 00:00 [received]
PHST- 2008/11/26 00:00 [revised]
PHST- 2008/11/26 00:00 [accepted]
PHST- 2008/12/27 09:00 [entrez]
PHST- 2008/12/27 09:00 [pubmed]
PHST- 2009/03/19 09:00 [medline]
AID - S0006-8993(08)02900-4 [pii]
AID - 10.1016/j.brainres.2008.11.099 [doi]
PST - ppublish
SO  - Brain Res. 2009 Feb 19;1255:170-9. doi: 10.1016/j.brainres.2008.11.099. Epub 2008 
      Dec 11.