PMID- 19110413
OWN - NLM
STAT- MEDLINE
DCOM- 20090526
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 24
IP  - 7
DP  - 2009 Mar 15
TI  - Development of an open stand-alone platform for regenerable automated 
      microarrays.
PG  - 2106-12
LID - 10.1016/j.bios.2008.11.005 [doi]
AB  - A novel automated chemiluminescence (CL) read-out system for analytical 
      flow-through microarrays based on multiplexed immunoassays has been developed. 
      The microarray chip reader (MCR 3) is designed as a stand-alone platform, with 
      the goal to quantify multiple analytes in complex matrices of food and liquid 
      samples for field analysis or for routine analytical laboratories. The CL 
      microarray platform is a self-contained system for the fully automated 
      multiplexed immunoanalysis: the microarray chip, the fluidic system and the 
      software module enable automated calibration and determination of analyte 
      concentrations during a whole working day. The detection of antibiotics in milk 
      was demonstrated to validate this device. There are few quantitative 
      multi-residue detection methods for routine analysis although the EU has defined 
      maximum residue limits (MRLs) for a number of antibacterial reagents. Therefore, 
      an automated multianalyte detection instrument is needed quantifying 
      simultaneously antibiotics within some minutes. Also regeneration is required to 
      avoid replacing the assay surface. The MCR 3 uses a microarray chip, which 
      consists of two channels for parallel measurement and regeneration. The 
      microarray chip is designed for parallel analysis of up to 13 different 
      antibiotics in milk applying an indirect competitive microarray immunoassay 
      (MIA). Microspotted antibiotics are directly coupled to epoxylated PEG surfaces. 
      As an initial example, penicillin G is quantified in milk on the MCR 3. The 
      penicillin G surface is regenerable for 47 measurement cycles per channel. A 
      limit of detection (LOD) of 1.1 microg/L is achieved by an assay time of 6 min.
FAU - Kloth, Katrin
AU  - Kloth K
AD  - Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische 
      Universitat Munchen, Marchioninistrasse 17, D-81377 Munchen, Germany.
FAU - Niessner, Reinhard
AU  - Niessner R
FAU - Seidel, Michael
AU  - Seidel M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081118
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Cattle
MH  - Equipment Reuse
MH  - Food Analysis/*instrumentation
MH  - Food Contamination/analysis
MH  - Immunoassay/*instrumentation
MH  - Luminescent Measurements/*instrumentation/methods
MH  - Microfluidic Analytical Techniques/*instrumentation/methods
MH  - Milk/*chemistry
MH  - Protein Array Analysis/*instrumentation
MH  - Systems Integration
EDAT- 2008/12/27 09:00
MHDA- 2009/05/27 09:00
CRDT- 2008/12/27 09:00
PHST- 2008/09/04 00:00 [received]
PHST- 2008/10/17 00:00 [revised]
PHST- 2008/11/05 00:00 [accepted]
PHST- 2008/12/27 09:00 [entrez]
PHST- 2008/12/27 09:00 [pubmed]
PHST- 2009/05/27 09:00 [medline]
AID - S0956-5663(08)00608-8 [pii]
AID - 10.1016/j.bios.2008.11.005 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2009 Mar 15;24(7):2106-12. doi: 10.1016/j.bios.2008.11.005. 
      Epub 2008 Nov 18.