PMID- 19111121
OWN - NLM
STAT- MEDLINE
DCOM- 20100309
LR  - 20081229
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 20
IP  - 12
DP  - 2008 Dec
TI  - [Study of the effect of p53 and Bcl-2 protein expression in myocardial apoptosis 
      in septic rats].
PG  - 733-6
AB  - OBJECTIVE: To explore the significance of p53 and Bcl-2 gene in myocardial 
      apoptosis in septic rats. METHODS: Adult male Sprague-Dawley (SD) rats were 
      randomly divided into normal control group (n=6), sham operation group (n=6) and 
      cecal ligation and puncture (CLP) group (n=24). The sepsis model was reproduced 
      by CLP. Rats in CLP group were divided into four subgroups according to the time 
      points of 3, 9, 12, and 24 hours after operation, with 6 rats in each subgroup, 
      and their hearts were examined according to the experimental protocol. Myocardial 
      apoptosis was detected with electron microscopy and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) technique. Bcl-2 and p53 
      protein expression was measured by immunohistochemistry method. RESULTS: 
      Myocardial apoptosis index (AI) in septic rats were increased, higher than that 
      in normal control group [(1.500+/- 0.141)%] and sham operation group 
      [(1.567+/-0.258)%, all P<0.05]. The myocardial AI peaked at 12 hours 
      [(55.633+/-2.073)%], and lowered at 24 hours [(33.683+/-2.070)a]. The expression 
      level of p53 protein in CLP group was lowest at 3 hours [(13.817+/-0.964)%], 
      peaked at 12 hours [(80.567+/-5.055)%], and higher than that in normal control 
      group [(0.617+/-0.232)%] and sham operation group [(0.600+/-0.297)%, all P<0.05 
      ]. The trend was parallel with that of the results of TUNEL. However, the 
      expression level of Bcl-2 protein peaked at 3 hours [(31.650+/-1.799)%], and was 
      lowest at 12 hours [(0.650+/-0.308)%], and lower than that in normal control 
      group [(47.017+/-0.691)%] and sham operation group [(46.817+/-0.567)%, all 
      P<0.05]. The trend was opposite with that of the results of TUNEL. CONCLUSION: 
      Myocardial apoptosis may be a mechanism of the pathogenesis of myocardial injury 
      in sepsis. The change in the modulating genes p53 and Bcl-2 of apoptosis may 
      serve as indicators of myocardial injury. p53 and Bcl-2 gene may be used as an 
      interventional means in sepsis to change its outcome.
FAU - Zhai, Cheng-Shun
AU  - Zhai CS
AD  - Medical Department, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou 510080, Guangdong, China.
FAU - Tang, Hao
AU  - Tang H
FAU - Liang, Yan-Bing
AU  - Liang YB
FAU - Chen, Zhi-Bin
AU  - Chen ZB
FAU - Li, Zhen-Yu
AU  - Li ZY
FAU - Ma, Zhong-Fu
AU  - Ma ZF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Disease Models, Animal
MH  - Male
MH  - Myocardium/metabolism/*pathology
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/*metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sepsis/metabolism/*pathology
MH  - Tumor Suppressor Protein p53/genetics/*metabolism
EDAT- 2008/12/30 09:00
MHDA- 2010/03/10 06:00
CRDT- 2008/12/30 09:00
PHST- 2008/12/30 09:00 [entrez]
PHST- 2008/12/30 09:00 [pubmed]
PHST- 2010/03/10 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008 Dec;20(12):733-6.