PMID- 19112916
OWN - NLM
STAT- MEDLINE
DCOM- 20100408
LR  - 20081230
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 29
IP  - 8
DP  - 2008 Aug
TI  - [Experimental study on the effect of sensitized sera on engraftment of 
      hematopoietic stem cells].
PG  - 531-4
AB  - OBJECTIVE: To evaluate the effect of sensitized sera on engraftment of 
      hematopoietic stem cells (HSCs) and its mechanism. METHODS: Bone marrow cells 
      (BMCs) from C57BL/6 mice were incubated with sensitized sera (group I) or normal 
      sera (group II), and then washed and transplanted into irradiated BALB/c mice. 
      The survival analysis and engraftment evaluation of the recipients were observed. 
      The incubated BMCs were bound with goat anti mouse IgG for and labeled with 
      Annexin V for apoptosis detection. RESULTS: Seven out of ten recipient mice in 
      group I died of bone marrow failure at day 10 after transplantation, while all of 
      those in group II were long-term survived. Engraftment assay showed recipients 
      blood count and BMCs progressively decreased along with time passing in group I; 
      in addition, the chimeric percentage of donor cells progressively decreased as 
      well. The percentage of BMCs binding with goat anti mouse IgG in group I or group 
      II were (90.3 +/- 5.1)% and (5.2 +/- 2.4)%, respectively, and the difference was 
      statistically significant (P < 0.01). However, no significant difference was 
      found in the apoptosis detection between the two groups (P > 0.05). CONCLUSION: 
      The engraftment capacity of HSCs is significantly impaired by sensitized sera, 
      the antibodies in sensitized sera may bind to antigens expressed on HSCs but do 
      not induce apoptosis.
FAU - Xu, Lu-Hong
AU  - Xu LH
AD  - Hematopoietic Stem Cell Transplantation Center, Department of Pediatrics, The 
      Second Affiliated Hospital, SUN Yat-sen University, Guangzhou 510120, China.
FAU - Fang, Jian-Pei
AU  - Fang JP
FAU - Xu, Hong-Gui
AU  - Xu HG
FAU - Weng, Wen-Jun
AU  - Weng WJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Annexin A5)
RN  - 0 (Immune Sera)
SB  - IM
MH  - Animals
MH  - Annexin A5/metabolism
MH  - Apoptosis
MH  - Bone Marrow Cells/metabolism/pathology
MH  - Cells, Cultured
MH  - *Hematopoietic Stem Cell Transplantation
MH  - *Immune Sera
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
EDAT- 2008/12/31 09:00
MHDA- 2010/04/09 06:00
CRDT- 2008/12/31 09:00
PHST- 2008/12/31 09:00 [entrez]
PHST- 2008/12/31 09:00 [pubmed]
PHST- 2010/04/09 06:00 [medline]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2008 Aug;29(8):531-4.