PMID- 19116689
OWN - NLM
STAT- MEDLINE
DCOM- 20090420
LR  - 20090227
IS  - 1573-6776 (Electronic)
IS  - 0141-5492 (Linking)
VI  - 31
IP  - 4
DP  - 2009 Apr
TI  - A suicidal DNA vaccine co-expressing two major membrane-associated proteins of 
      porcine reproductive and respiratory syndrome virus antigens induce protective 
      responses.
PG  - 509-18
LID - 10.1007/s10529-008-9905-0 [doi]
AB  - We constructed a suicidal DNA vaccine pSFV-ORF5m/ORF6 co-expressing GP5m (a 
      modified GP5) and M proteins of porcine reproductive and respiratory syndrome 
      virus (PRRSV). In mice immunization, specific immune responses were elicited by 
      the suicidal DNA vaccine pSFV-ORF5m/ORF6. The immunogenicity and protective 
      efficiency was then evaluated in piglets immunized with pSFV-ORF5m/ORF6 before 
      virus challenge: PRRSV-specific neutralizing antibodies and lymphocyte 
      proliferative responses were developed. Post-PRRSV challenge, these immune 
      responses were further boosted and partial protection was obtained.
FAU - Jiang, Yunbo
AU  - Jiang Y
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, 1 Shi-zi-shan Street, 430070 Wuhan, Hubei, People's Republic of 
      China.
FAU - Fang, Liurong
AU  - Fang L
FAU - Xiao, Shaobo
AU  - Xiao S
FAU - Li, Bin
AU  - Li B
FAU - Pan, Yongfei
AU  - Pan Y
FAU - Luo, Rui
AU  - Luo R
FAU - Chen, Huanchun
AU  - Chen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081231
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (Antibodies, Viral)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Kidney/virology
MH  - Lung/pathology/virology
MH  - Lymph Nodes/virology
MH  - Neutralization Tests
MH  - Palatine Tonsil/virology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/immunology
MH  - Spleen/virology
MH  - Swine
MH  - Vaccines, DNA/*genetics/*immunology
MH  - Viral Envelope Proteins/genetics/*immunology
MH  - Viral Matrix Proteins/genetics/*immunology
EDAT- 2009/01/01 09:00
MHDA- 2009/04/21 09:00
CRDT- 2009/01/01 09:00
PHST- 2008/08/26 00:00 [received]
PHST- 2008/12/05 00:00 [accepted]
PHST- 2008/12/04 00:00 [revised]
PHST- 2009/01/01 09:00 [entrez]
PHST- 2009/01/01 09:00 [pubmed]
PHST- 2009/04/21 09:00 [medline]
AID - 10.1007/s10529-008-9905-0 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2009 Apr;31(4):509-18. doi: 10.1007/s10529-008-9905-0. Epub 2008 
      Dec 31.