PMID- 19118097
OWN - NLM
STAT- MEDLINE
DCOM- 20090413
LR  - 20200930
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 296
IP  - 3
DP  - 2009 Mar
TI  - Protein synthesis and the expression of growth-related genes are altered by 
      running in human vastus lateralis and soleus muscles.
PG  - R708-14
LID - 10.1152/ajpregu.90906.2008 [doi]
AB  - Recent evidence suggests aerobic exercise may help preserve soleus muscle mass 
      during unloading. The purpose of this investigation was to examine the 
      muscle-specific metabolic response to running as it relates to muscle growth. 
      Mixed-muscle protein synthesis [fractional synthetic rate (FSR)] and gene 
      expression (GE) were examined in the vastus lateralis (VL) and soleus (SOL) 
      muscles from eight men (26 +/- 2 yr; Vo(2max) 63 +/- 2 ml.kg(-1).min(-1)) before 
      and after a 45-min level-grade treadmill run at 77 +/- 1% intensity. Muscle 
      glycogen utilization was similar between muscles. Resting FSR was similar between 
      the VL (0.080 +/- 0.007 %/h) and SOL (0.086 +/- 0.008 %/h) and was higher (P < 
      0.05) 24 h postexercise compared with rest for both muscles. The absolute change 
      in FSR was not different between muscles (0.030 +/- 0.007 vs. 0.037 +/- 0.012 %/h 
      for VL and SOL). At baseline, myostatin GE was approximately twofold higher (P < 
      0.05) in SOL compared with VL, while no other muscle-specific differences in GE 
      were present. After running, myostatin GE was suppressed (P < 0.05) in both 
      muscles at 4 h and was higher (P < 0.05) than baseline at 24 h for VL only. 
      Muscle regulatory factor 4 mRNA was elevated (P < 0.05) at 4 h in both SOL and 
      VL; MyoD and peroxisome-proliferator-activated receptor-gamma coactivator-1alpha 
      (PGC-1alpha) were higher (P < 0.05) at 4 h, and forkhead box [FOXO]3A was higher 
      at 24 h in SOL only, while muscle-RING-finger protein-1 (MuRF-1) was higher (P < 
      0.05) at 4 h in VL only. Myogenin and atrogin-1 GE were unaltered. The similar 
      increases between muscles in FSR support running as part of the exercise 
      countermeasure to preserve soleus mass during unloading. The subtle differences 
      in GE suggest a potential mechanism for muscle-specific adaptations to chronic 
      run training.
FAU - Harber, Matthew P
AU  - Harber MP
AD  - Ball State Univ., Muncie, IN 47306, USA. mharber@bsu.edu
FAU - Crane, Justin D
AU  - Crane JD
FAU - Dickinson, Jared M
AU  - Dickinson JM
FAU - Jemiolo, Bozena
AU  - Jemiolo B
FAU - Raue, Ulrika
AU  - Raue U
FAU - Trappe, Todd A
AU  - Trappe TA
FAU - Trappe, Scott W
AU  - Trappe SW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081231
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Muscle Proteins)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - 63231-63-0 (RNA)
RN  - 9005-79-2 (Glycogen)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Adult
MH  - Glycogen/metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*biosynthesis/*genetics
MH  - Male
MH  - Muscle Contraction/physiology
MH  - Muscle Fibers, Skeletal/metabolism
MH  - Muscle Proteins/*biosynthesis/*genetics
MH  - Muscle, Skeletal/anatomy & histology/*metabolism/*physiology
MH  - Myosin Heavy Chains/biosynthesis/genetics
MH  - Oxygen Consumption/physiology
MH  - Phenylalanine/blood
MH  - RNA/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Running/*physiology
EDAT- 2009/01/02 09:00
MHDA- 2009/04/14 09:00
CRDT- 2009/01/02 09:00
PHST- 2009/01/02 09:00 [entrez]
PHST- 2009/01/02 09:00 [pubmed]
PHST- 2009/04/14 09:00 [medline]
AID - 90906.2008 [pii]
AID - 10.1152/ajpregu.90906.2008 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2009 Mar;296(3):R708-14. doi: 
      10.1152/ajpregu.90906.2008. Epub 2008 Dec 31.