PMID- 19118576
OWN - NLM
STAT- MEDLINE
DCOM- 20091007
LR  - 20171116
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 122
IP  - 1
DP  - 2009 Jan 29
TI  - Premature senescence of T lymphocytes from patients with beta-thalassemia major.
PG  - 84-8
LID - 10.1016/j.imlet.2008.12.003 [doi]
AB  - Several researches have demonstrated a suppressed cell mediated immunity in 
      patients with beta-thalassemia major. To know whether the premature aging of T 
      cells is involved in abnormalities of cell mediated immunity, the biomarkers of 
      immunosenescence including telomerase activity, apoptosis, and the expression of 
      CD28 and CD95 were evaluated in T lymphocytes from beta-thalassemia major 
      patients. The ex vivo spontaneous apoptosis in CD4(+) or CD8(+) T cells from 
      patients and healthy subjects was assessed by an in situ TdT mediated dUTP-biotin 
      nick end labelling (TUNEL) assay after 24h incubation in medium. Flow cytometric 
      data revealed that lymphocytes from beta-thalassemia patients were resistant to 
      spontaneous apoptosis compared to the normal lymphocytes. Moreover, the 
      percentages of TUNEL(+)CD4(+) or TUNEL(+)CD8(+) T cells from patients were 
      significantly lower than those control cells. Quantitative determination of 
      telomerase activity in resting and activated T cells was performed using the 
      Telomeric Repeat Amplification Protocol (TRAP). The results showed a decreased 
      telomerase activity of activated T cells in patients with thalassemia major 
      compared to that in healthy controls. However, the percentages of CD8(+)CD28(-) 
      and CD3(+)CD95(+) T lymphocytes were significantly higher in thalassemia 
      patients, indicating the phenotypes associated with senescent T lymphocytes. 
      These data provide evidences for the occurrence of accelerated aging of T cells 
      in beta-thalassemia major; possibly result in abnormal T cell function leading to 
      suppressed cell mediated immunity.
FAU - Gharagozloo, Marjan
AU  - Gharagozloo M
AD  - Department of Immunology, Isfahan University of Medical Sciences, Isfahan, Iran. 
      gharagozlo@sums.ac.ir
FAU - Bagherpour, Bahram
AU  - Bagherpour B
FAU - Tahanian, Mahmoud
AU  - Tahanian M
FAU - Oreizy, Farzad
AU  - Oreizy F
FAU - Amirghofran, Zahra
AU  - Amirghofran Z
FAU - Sadeghi, Hamid Mir Mohammad
AU  - Sadeghi HM
FAU - Hourfar, Hamid
AU  - Hourfar H
FAU - Moayedi, Behjat
AU  - Moayedi B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081230
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Biomarkers)
RN  - 0 (CD28 Antigens)
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (fas Receptor)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Apoptosis/immunology
MH  - Biomarkers/blood
MH  - CD28 Antigens/genetics/immunology/*metabolism
MH  - CD4 Antigens
MH  - CD8 Antigens
MH  - Cell Separation
MH  - Cellular Senescence/genetics/immunology
MH  - Child
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Oxidative Stress
MH  - T-Lymphocytes/immunology/*metabolism/pathology
MH  - Telomerase/genetics/*immunology/metabolism
MH  - beta-Thalassemia/blood/enzymology/*immunology/physiopathology
MH  - fas Receptor/genetics/immunology/*metabolism
EDAT- 2009/01/03 09:00
MHDA- 2009/10/08 06:00
CRDT- 2009/01/03 09:00
PHST- 2008/09/07 00:00 [received]
PHST- 2008/11/27 00:00 [revised]
PHST- 2008/12/01 00:00 [accepted]
PHST- 2009/01/03 09:00 [entrez]
PHST- 2009/01/03 09:00 [pubmed]
PHST- 2009/10/08 06:00 [medline]
AID - S0165-2478(08)00270-8 [pii]
AID - 10.1016/j.imlet.2008.12.003 [doi]
PST - ppublish
SO  - Immunol Lett. 2009 Jan 29;122(1):84-8. doi: 10.1016/j.imlet.2008.12.003. Epub 
      2008 Dec 30.