PMID- 19118897
OWN - NLM
STAT- MEDLINE
DCOM- 20090427
LR  - 20191210
IS  - 1873-5835 (Electronic)
IS  - 0145-2126 (Linking)
VI  - 33
IP  - 6
DP  - 2009 Jun
TI  - Validation of a new three-color fluorescence in situ hybridization (FISH) method 
      to detect CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocations.
PG  - 843-6
LID - 10.1016/j.leukres.2008.11.016 [doi]
AB  - The PDGRFA locus has become a gene of interest based on mutational activation in 
      various myeloid neoplasms and the availability of targeted therapies (i.e., 
      imatinib mesylate) to its overexpression. We studied a new FISH method to detect 
      CHIC2 deletion, FIP1L1/PDGFRA fusion and PDGFRA translocation in patients with 
      myeloid neoplasms associated with eosinophilia. A total of 46 specimens were 
      studied, including 15 from patients with a CHIC2 abnormality and six patients 
      with an abnormality involving PDGFRA. Our results revealed this new FISH assay 
      accurately detects these abnormalities and will be a useful clinical test for 
      patients with myeloid neoplasms and eosinophilia.
FAU - Fink, Stephanie R
AU  - Fink SR
AD  - Division of Laboratory Genetics, Mayo Clinic, Rochester, MN 55905, USA.
FAU - Belongie, Kimberly J
AU  - Belongie KJ
FAU - Paternoster, Sarah F
AU  - Paternoster SF
FAU - Smoley, Stephanie A
AU  - Smoley SA
FAU - Pardanani, Animesh D
AU  - Pardanani AD
FAU - Tefferi, Ayalew
AU  - Tefferi A
FAU - Van Dyke, Daniel L
AU  - Van Dyke DL
FAU - Ketterling, Rhett P
AU  - Ketterling RP
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20081231
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (CHIC2 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Transcription Factors)
RN  - 0 (mRNA Cleavage and Polyadenylation Factors)
RN  - EC 2.7.10.1 (FIP1L1-PDGFRA fusion protein, human)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor alpha)
SB  - IM
MH  - DNA-Binding Proteins/*genetics
MH  - *Gene Deletion
MH  - *Gene Fusion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods/*standards
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Receptor, Platelet-Derived Growth Factor alpha/*genetics
MH  - Sensitivity and Specificity
MH  - Transcription Factors/*genetics
MH  - *Translocation, Genetic
MH  - mRNA Cleavage and Polyadenylation Factors/*genetics
EDAT- 2009/01/03 09:00
MHDA- 2009/04/28 09:00
CRDT- 2009/01/03 09:00
PHST- 2008/08/28 00:00 [received]
PHST- 2008/11/13 00:00 [revised]
PHST- 2008/11/14 00:00 [accepted]
PHST- 2009/01/03 09:00 [entrez]
PHST- 2009/01/03 09:00 [pubmed]
PHST- 2009/04/28 09:00 [medline]
AID - S0145-2126(08)00503-1 [pii]
AID - 10.1016/j.leukres.2008.11.016 [doi]
PST - ppublish
SO  - Leuk Res. 2009 Jun;33(6):843-6. doi: 10.1016/j.leukres.2008.11.016. Epub 2008 Dec 
      31.