PMID- 1911989
OWN - NLM
STAT- MEDLINE
DCOM- 19911121
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 3
IP  - 3
DP  - 1991 Jul
TI  - Molecular diagnosis of alcelaphine herpesvirus (malignant catarrhal fever) 
      infections by nested amplification of viral DNA in bovine blood buffy coat 
      specimens.
PG  - 193-8
AB  - A fragment of alcelaphine herpesvirus-1 (AHV-1; malignant catarrhal fever) DNA 
      was subcloned into pUC 18 and sequenced. The subclone hybridized strongly to 
      AHV-1 DNA, weakly to alcelaphine herpesvirus-2 (AHV-2) DNA, and not at all to DNA 
      from bovine herpesvirus-1 (BHV-1; infectious bovine rhinotracheitis [IBR] virus), 
      bovine herpesvirus-2 (BHV-2; bovine herpes mamillitis [BHM] virus), and bovine 
      herpesvirus-4 (BHV-4; isolate DN599). A 2-stage (nested) polymerase chain 
      reaction (PCR) diagnostic test was devised based on a portion of the subcloned 
      AHV-1 DNA sequence. First and second stage amplified AHV-1 DNA targets were 487 
      and 172 base pairs (bp) in length, respectively. Unique Pvu II and Stu I 
      restriction endonuclease cleavage sites confirmed the identity of amplified AHV-1 
      DNA. Five AHV-1 and 2 AHV-2 isolates were identically and specifically PCR 
      positive. BHV-1, BHV-2, and BHV-4 viruses were negative by the same procedure. As 
      little as 0.01 TCID50 AHV-1 was detected using the nested amplification 
      procedure. Simple methods of buffy coat isolation from bovine blood were employed 
      to prepare specimens for PCR. An AHV-1-infected calf was PCR positive from 3 to 
      77 days postinoculation (PI), with rising seroconversion first noted 14 days PI. 
      The AHV-1 DNA sequence was 62% homologous to a portion of the Epstein-Barr virus 
      genome. The nested PCR procedure may improve the viral diagnosis of clinical and 
      subclinical alcelaphine herpesvirus infections.
FAU - Katz, J
AU  - Katz J
AD  - Diagnostic Virology Laboratory, US Department of Agriculture, Ames, IA 50010.
FAU - Seal, B
AU  - Seal B
FAU - Ridpath, J
AU  - Ridpath J
LA  - eng
SI  - GENBANK/M55380
PT  - Journal Article
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cloning, Molecular
MH  - DNA, Viral/*analysis/chemistry
MH  - Herpesviridae/*genetics
MH  - Malignant Catarrh/*microbiology
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction
MH  - Restriction Mapping
MH  - *Ruminants
EDAT- 1991/07/01 00:00
MHDA- 1991/07/01 00:01
CRDT- 1991/07/01 00:00
PHST- 1991/07/01 00:00 [pubmed]
PHST- 1991/07/01 00:01 [medline]
PHST- 1991/07/01 00:00 [entrez]
AID - 10.1177/104063879100300301 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 1991 Jul;3(3):193-8. doi: 10.1177/104063879100300301.