PMID- 19120029
OWN - NLM
STAT- MEDLINE
DCOM- 20090211
LR  - 20190917
IS  - 1608-3040 (Electronic)
IS  - 0006-2979 (Linking)
VI  - 73
IP  - 11
DP  - 2008 Nov
TI  - Antibodies against DNA hydrolyze DNA and RNA.
PG  - 1242-53
AB  - In this work, rabbits were immunized with a high polymer DNA complexed with 
      methylated BSA (mBSA) and by mBSA. It is shown that electrophoretically 
      homogeneous preparations of polyclonal antibodies (Ab) from non-immunized rabbits 
      and animals immunized by mBSA do not exhibit catalytic activity. Ab from the 
      blood of rabbits immunized with the DNA-mBSA complex hydrolyzed poly(C) and 
      different RNAs with efficiency exceeding that towards DNA by approximately 3-4 
      orders of magnitude. Affinity chromatography of the IgG on DNA cellulose 
      separated the Ab into fractions hydrolyzing both RNA and DNA, and for the first 
      time fractions that hydrolyze only RNA were found. Kinetic parameters that 
      characterize the RNA and DNA hydrolysis by initial Ab preparations and their 
      fractions obtained by separation on an affinity sorbent are compared.
FAU - Krasnorutskii, M A
AU  - Krasnorutskii MA
AD  - Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the 
      Russian Academy of Sciences, Novosibirsk, 630090, Russia.
FAU - Buneva, V N
AU  - Buneva VN
FAU - Nevinsky, G A
AU  - Nevinsky GA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry (Mosc)
JT  - Biochemistry. Biokhimiia
JID - 0376536
RN  - 0 (Antibodies, Catalytic)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.- (Deoxyribonucleases)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Antibodies, Catalytic/chemistry/immunology/isolation & purification/*metabolism
MH  - Cattle
MH  - Chromatography, Affinity
MH  - DNA/immunology/*metabolism
MH  - Deoxyribonucleases/chemistry/immunology/isolation & purification/*metabolism
MH  - Hydrolysis
MH  - Kinetics
MH  - RNA/immunology/*metabolism
MH  - Rabbits
MH  - Ribonucleases/chemistry/immunology/isolation & purification/*metabolism
MH  - Serum Albumin, Bovine/immunology/metabolism
EDAT- 2009/01/06 09:00
MHDA- 2009/02/12 09:00
CRDT- 2009/01/06 09:00
PHST- 2009/01/06 09:00 [entrez]
PHST- 2009/01/06 09:00 [pubmed]
PHST- 2009/02/12 09:00 [medline]
AID - BCM73111547 [pii]
AID - 10.1134/s0006297908110114 [doi]
PST - ppublish
SO  - Biochemistry (Mosc). 2008 Nov;73(11):1242-53. doi: 10.1134/s0006297908110114.