PMID- 19123232
OWN - NLM
STAT- MEDLINE
DCOM- 20090713
LR  - 20240419
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Print)
IS  - 0736-0266 (Linking)
VI  - 27
IP  - 7
DP  - 2009 Jul
TI  - Effects of multiple chondroitinase ABC applications on tissue engineered 
      articular cartilage.
PG  - 949-56
LID - 10.1002/jor.20821 [doi]
AB  - Increasing tensile properties and collagen content is a recognized need in 
      articular cartilage tissue engineering. This study tested the hypothesis that 
      multiple applications of chondroitinase ABC (C-ABC), a glycosaminoglycan (GAG) 
      degrading enzyme, could increase construct tensile properties in a scaffold-less 
      approach for articular cartilage tissue engineering. Developing constructs were 
      treated with C-ABC at 2 weeks, 4 weeks, or both 2 and 4 weeks. At 4 and 6 weeks, 
      construct sulfated GAG composition, collagen composition, and compressive and 
      tensile biomechanical properties were assessed, along with immunohistochemistry 
      (IHC) for collagens type I, II, and VI, and the proteoglycan decorin. At 6 weeks, 
      the tensile modulus and ultimate tensile strength of the group treated at both 2 
      and 4 weeks were significantly increased over controls by 78% and 64%, reaching 
      values of 3.4 and 1.4 MPa, respectively. Collagen concentration also increased 
      43%. Further, groups treated at either 2 weeks or 4 weeks alone also had 
      increased tensile stiffness compared to controls. Surprisingly, though GAG was 
      depleted in the treated groups, by 6 weeks there were no significant differences 
      in compressive stiffness. IHC showed abundant collagen type II and VI in all 
      groups, with no collagen type I. Further, decorin staining was reduced following 
      C-ABC treatment, but returned during subsequent culture. The results support the 
      use of C-ABC in cartilage tissue engineering for increasing tensile properties.
FAU - Natoli, Roman M
AU  - Natoli RM
AD  - Department of Bioengineering, Rice University, 6100 Main Street, Keck Hall, Suite 
      116, Houston, Texas 77005, USA.
FAU - Responte, Donald J
AU  - Responte DJ
FAU - Lu, Benjamin Y
AU  - Lu BY
FAU - Athanasiou, Kyriacos A
AU  - Athanasiou KA
LA  - eng
GR  - R01 AR053286/AR/NIAMS NIH HHS/United States
GR  - R01 AR053286-03/AR/NIAMS NIH HHS/United States
GR  - R01AR053286/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type II)
RN  - 0 (Collagen Type IV)
RN  - 0 (Decorin)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Proteoglycans)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*cytology/physiology
MH  - Cattle
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology/*drug effects
MH  - Chondroitin ABC Lyase/*pharmacology
MH  - Collagen Type I/metabolism
MH  - Collagen Type II/metabolism
MH  - Collagen Type IV/metabolism
MH  - Compressive Strength/physiology
MH  - Decorin
MH  - Extracellular Matrix Proteins/metabolism
MH  - Glycosaminoglycans/metabolism
MH  - Models, Biological
MH  - Proteoglycans/metabolism
MH  - Tensile Strength/physiology
MH  - Tissue Engineering/*methods
PMC - PMC2819396
MID - NIHMS173594
EDAT- 2009/01/06 09:00
MHDA- 2009/07/14 09:00
PMCR- 2010/02/10
CRDT- 2009/01/06 09:00
PHST- 2009/01/06 09:00 [entrez]
PHST- 2009/01/06 09:00 [pubmed]
PHST- 2009/07/14 09:00 [medline]
PHST- 2010/02/10 00:00 [pmc-release]
AID - 10.1002/jor.20821 [doi]
PST - ppublish
SO  - J Orthop Res. 2009 Jul;27(7):949-56. doi: 10.1002/jor.20821.