PMID- 19123331
OWN - NLM
STAT- MEDLINE
DCOM- 20100810
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 28
IP  - 10
DP  - 2008 Oct
TI  - [Effect of Astragalus and Arctium in different combinations on reactive oxygen 
      species content and nuclear transcription factor kappaB expression in renal 
      tissue of streptozotocin rats].
PG  - 917-20
AB  - OBJECTIVE: To investigate the reactive oxygen species (ROS) content and nuclear 
      transcription factor-kappa B expression (NF-kappaB) in renal tissue of diabetic 
      rats and the effect of Astragalus and Arctium in combination on them. METHODS: 
      Streptozotocin (STZ)-induced diabetic model rats were established. Astragalus and 
      Arctium in different dosages (low, moderate, high) were combined after orthogonal 
      design and administered respectively to the model rats via gastrogavage for 4 or 
      8 weeks. Content of ROS in the renal tissue was detected by flow cytometry and 
      expression of functional NF-kappaB p65 was assessed by Western blot at the 4th 
      and 8th weekends (4th week and 8th week). RESULTS: ROS content of renal tissue in 
      the model rats was 36.55 +/- 7.46% at the 4th week and 31.91 +/- 5.83% at the 8th 
      week, NF-kappaB p65 expression was 165.00 +/- 3.14 at the 4th week and 214.00 +/- 
      5.11 at the 8th week, all higher than those in normal rats (6.21 +/- 1.83% and 
      129.00 +/- 1.58 at the 4th week, 6.95 +/- 1.41% and 148.00 +/- 2.32 at the 8th 
      week) respectively. The combined use of Astragalus and Arctium showed decreasing 
      effects on both indexes significantly, and the decreasing effects of the 
      combination with moderate and high dose Astragalus were better than those with 
      low dose, with the details as follows: those of ROS at the 4th week were 11.43 
      +/- 2.42%, 18.37 +/- 7.58% and 22.10 +/- 4.71% for high, moderate and low dose 
      Astragalus combination respectively (same hereinafter), at the 8th week 12.55 
      +/-4.40%, 19.15 +/- 6.64% and 23.48 +/- 3.13%; and for NF-kappaB p65 expression 
      at the 8th week, 185.00 +/- 6.99, 183.00 +/- 3.89 and 194.00 +/- 4.98 
      respectively. CONCLUSION: Combined use of Astragalus and Arctium may ameliorate 
      the condition of diabetic nephropathy by inhibiting the activation of the 
      ROS-NF-kappaB signal passage.
FAU - Wang, Wei-Wei
AU  - Wang WW
AD  - The 455th Hospital of PLA, Shanghai. wangweiwei1232@163.com
FAU - Chen, Yi-Ping
AU  - Chen YP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 5W494URQ81 (Streptozocin)
SB  - IM
MH  - Animals
MH  - Arctium/*chemistry
MH  - Astragalus Plant/*chemistry
MH  - Diabetic Nephropathies/chemically induced/*drug therapy/*metabolism
MH  - Disease Models, Animal
MH  - Drug Therapy, Combination
MH  - Gene Expression/*drug effects
MH  - Humans
MH  - NF-kappa B/*genetics/metabolism
MH  - Plant Extracts/*administration & dosage
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/*metabolism
MH  - Streptozocin/adverse effects
EDAT- 2009/01/07 09:00
MHDA- 2010/08/11 06:00
CRDT- 2009/01/07 09:00
PHST- 2009/01/07 09:00 [entrez]
PHST- 2009/01/07 09:00 [pubmed]
PHST- 2010/08/11 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Oct;28(10):917-20.