PMID- 19123478
OWN - NLM
STAT- MEDLINE
DCOM- 20090319
LR  - 20160303
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
VI  - 124
IP  - 8
DP  - 2009 Apr 15
TI  - Hepatocarcinogenesis in mice with a conditional knockout of the hepatocyte growth 
      factor receptor c-Met.
PG  - 1767-72
LID - 10.1002/ijc.24167 [doi]
AB  - The receptor for the hepatocyte growth factor/scatter factor (HGF/SF), c-Met, 
      plays a role in tumour promotion, progression and metastasis. In this study, we 
      analysed chemically induced hepatocarcinogenesis in mice lacking a functional HGF 
      receptor in their liver. Control and c-Met deficient mice were injected with a 
      single dose of N-nitrosodiethylamine (DEN, 90 mICROg/g b.wt.) at 6 weeks of age 
      and mice were subsequently kept on a phenobarbital (PB) containing diet (0.05%) 
      for 35 weeks or on control diet. At the end of the experiment, the carcinogenic 
      response in liver of the animals was monitored. Conditional c-met knockout (KO) 
      mice showed a higher prevalence of macroscopically visible liver tumours and of 
      glutamine synthetase positive and glucose-6-phosphatase deficient lesions in 
      liver. Tumour promotion by PB led to significant increases in the number of 
      preneoplastic and neoplastic lesions in liver of both wild-type and c-met 
      knockout mice, with only minor differences in response. Our results indicate that 
      a defect in c-Met-mediated signaling increases chemically induced tumour 
      initiation in liver but does not significantly affect PB-mediated tumour 
      promotion.
FAU - Marx-Stoelting, Philip
AU  - Marx-Stoelting P
AD  - Department of Toxicology, Institute of Pharmacology and Toxicology, University of 
      Tubingen, Tubingen, Germany.
FAU - Borowiak, Malgorzata
AU  - Borowiak M
FAU - Knorpp, Thomas
AU  - Knorpp T
FAU - Birchmeier, Carmen
AU  - Birchmeier C
FAU - Buchmann, Albrecht
AU  - Buchmann A
FAU - Schwarz, Michael
AU  - Schwarz M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 3IQ78TTX1A (Diethylnitrosamine)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 3.1.3.9 (Glucose-6-Phosphatase)
RN  - EC 6.3.1.2 (Glutamate-Ammonia Ligase)
RN  - YQE403BP4D (Phenobarbital)
SB  - IM
MH  - Animals
MH  - Diethylnitrosamine/pharmacology
MH  - Glucose-6-Phosphatase/metabolism
MH  - Glutamate-Ammonia Ligase/metabolism
MH  - Liver/drug effects/metabolism
MH  - Liver Neoplasms/chemically induced/*genetics
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Organ Size
MH  - Phenobarbital/toxicity
MH  - Phenotype
MH  - Proto-Oncogene Proteins c-met/*metabolism
MH  - Signal Transduction
MH  - Time Factors
EDAT- 2009/01/07 09:00
MHDA- 2009/03/20 09:00
CRDT- 2009/01/07 09:00
PHST- 2009/01/07 09:00 [entrez]
PHST- 2009/01/07 09:00 [pubmed]
PHST- 2009/03/20 09:00 [medline]
AID - 10.1002/ijc.24167 [doi]
PST - ppublish
SO  - Int J Cancer. 2009 Apr 15;124(8):1767-72. doi: 10.1002/ijc.24167.