PMID- 19123682
OWN - NLM
STAT- MEDLINE
DCOM- 20090319
LR  - 20191210
IS  - 1083-3668 (Print)
IS  - 1083-3668 (Linking)
VI  - 13
IP  - 6
DP  - 2008 Nov-Dec
TI  - Bioimaging assessment and effect of skin wound healing using bone-marrow-derived 
      mesenchymal stromal cells with the artificial dermis in diabetic rats.
PG  - 064036
LID - 10.1117/1.3042266 [doi]
AB  - We investigate the relationship between the fate and healing effect of 
      transplanted mesenchymal stromal cells (MSCs) in a rat diabetic skin wound model. 
      Rats are treated with streptozotocin to induce diabetic conditions. A 
      full-thickness skin defect is surgically made on the head of diabetic rats, and 
      covered with an artificial dermis impregnated with either bone marrow cells 
      (BMCs) or bone-marrow-derived MSCs from firefly luciferase (luc) transgenic (Tg) 
      rats. Wound healing is evaluated using planimetry and immunohistochemistry, and 
      the fate of transplanted MSCs is determined using in-vivo luminescent imaging. 
      The diabetic wound treated with MSCs-impregnated artificial dermis is 
      significantly smaller than that treated with artificial dermis alone at 1 week 
      postoperation. Photons of luc+ MSCs are detected at the transplanted site during 
      healing (3 weeks), whereas those of luc+ MSCs are depleted only after 1 week 
      postimplantation. Immunohistochemistry at the healing site treated with MSCs 
      demonstrates that CD31+ vessels increase with expression of vascular endothelial 
      growth factor, suggesting that MSCs accelerate angiogenesis. These findings 
      suggest that transplanted MSCs could be retained at wound sites during the 
      healing process in a diabetic rat model, and subsequently promote wound healing 
      through angiogenesis.
FAU - Inoue, Hirokazu
AU  - Inoue H
AD  - Jichi Medical University, Division of Organ Replacement Research, Center for 
      Molecular Medicine and Department of Orthopedics, Tochigi 329-0498, Japan.
FAU - Murakami, Takashi
AU  - Murakami T
FAU - Ajiki, Takashi
AU  - Ajiki T
FAU - Hara, Mayumi
AU  - Hara M
FAU - Hoshino, Yuichi
AU  - Hoshino Y
FAU - Kobayashi, Eiji
AU  - Kobayashi E
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation
MH  - Diabetes Complications/*pathology/*surgery
MH  - *Disease Models, Animal
MH  - Luminescent Measurements/methods
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/pathology
MH  - Microscopy, Fluorescence/methods
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Skin Ulcer/*pathology/*surgery
MH  - *Skin, Artificial
MH  - Treatment Outcome
MH  - *Wound Healing
EDAT- 2009/01/07 09:00
MHDA- 2009/03/20 09:00
CRDT- 2009/01/07 09:00
PHST- 2009/01/07 09:00 [entrez]
PHST- 2009/01/07 09:00 [pubmed]
PHST- 2009/03/20 09:00 [medline]
AID - 10.1117/1.3042266 [doi]
PST - ppublish
SO  - J Biomed Opt. 2008 Nov-Dec;13(6):064036. doi: 10.1117/1.3042266.