PMID- 19124280
OWN - NLM
STAT- MEDLINE
DCOM- 20090428
LR  - 20131121
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 877
IP  - 3
DP  - 2009 Jan 15
TI  - Development of an LC-ESI-MS/MS method for the determination of histamine: 
      application to the quantitative measurement of histamine degranulation by KU812 
      cells.
PG  - 207-12
LID - 10.1016/j.jchromb.2008.12.012 [doi]
AB  - A rapid, simple, and sensitive liquid chromatography-electrospray ionization 
      tandem mass spectrometry (LC-ESI-MS/MS) method was developed for the 
      identification and quantification of histamine without a previous derivatization 
      step or the addition of general ion-pairing reagents to the mobile phase. This 
      method was used to measure histamine release following degranulation of KU812 
      human basophilic cells, using pyrazol as an internal standard. Analyses were 
      performed on an LC system employing a Cosmosil 5C(18) PAQ column and an isocratic 
      elution with methanol-0.005% trifluoroacetic acid (1:1) at a flow rate of 0.2 
      mL/min. A triple-quadrupole mass spectrometer, equipped with an electrospray 
      ionization interface was employed, operating in the positive ion mode. The 
      retention time of histamine and the internal standard were 4.0 and 5.0 min, 
      respectively. The relative standard deviations (R.S.D.s) of the retention time 
      and peak area were between 0.47% and 2.03%. Micropipette tip solid-phase 
      extraction (SPE) using LooseTip C(18) allowed for not only rapid sample 
      preparation, but also decreased suppression effects, improving peak shape. This 
      method was used to evaluate the anti-allergic effects of compounds contained in 
      Taxus yunnanensis extracts. Four constituents that were isolated from the wood 
      extracts of T. yunnanensis and sodium cromoglicate, which is used as a first line 
      anti-allergic drug, were tested in an in vitro histamine release inhibition 
      assay. Of these compounds, taxiresinol and isotaxiresinol were more inhibitory 
      than sodium cromoglicate.
FAU - Koyama, Junko
AU  - Koyama J
AD  - Kobe Pharmaceutical University, Higashinada, Kobe 658-8558, Japan. 
      j-koyama@kobepharma-u.ac.jp
FAU - Takeuchi, Atsuko
AU  - Takeuchi A
FAU - Tode, Chisato
AU  - Tode C
FAU - Shimizu, Maki
AU  - Shimizu M
FAU - Morita, Izumi
AU  - Morita I
FAU - Nobukawa, Machiko
AU  - Nobukawa M
FAU - Nobukawa, Makiko
AU  - Nobukawa M
FAU - Kobayashi, Norihiro
AU  - Kobayashi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081211
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Pyrazoles)
RN  - 3QD5KJZ7ZJ (pyrazole)
RN  - 820484N8I3 (Histamine)
RN  - Q2WXR1I0PK (Cromolyn Sodium)
SB  - IM
MH  - Anti-Allergic Agents/pharmacology
MH  - Cell Degranulation/*drug effects
MH  - Cell Line
MH  - Chromatography, Liquid
MH  - Cromolyn Sodium/pharmacology
MH  - Histamine/*analysis
MH  - Histamine Release/*drug effects
MH  - Humans
MH  - Least-Squares Analysis
MH  - Plant Extracts/*pharmacology
MH  - Pyrazoles/analysis
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solid Phase Microextraction
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
MH  - Taxus/*chemistry
MH  - Wood/chemistry
EDAT- 2009/01/07 09:00
MHDA- 2009/04/29 09:00
CRDT- 2009/01/07 09:00
PHST- 2008/10/23 00:00 [received]
PHST- 2008/12/04 00:00 [revised]
PHST- 2008/12/05 00:00 [accepted]
PHST- 2009/01/07 09:00 [entrez]
PHST- 2009/01/07 09:00 [pubmed]
PHST- 2009/04/29 09:00 [medline]
AID - S1570-0232(08)00908-2 [pii]
AID - 10.1016/j.jchromb.2008.12.012 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15;877(3):207-12. doi: 
      10.1016/j.jchromb.2008.12.012. Epub 2008 Dec 11.