PMID- 1912562
OWN - NLM
STAT- MEDLINE
DCOM- 19911114
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 78
IP  - 7
DP  - 1991 Oct 1
TI  - Human platelet-specific antigen, Siba, is associated with the molecular weight 
      polymorphism of glycoprotein Ib alpha.
PG  - 1722-9
AB  - Platelet-specific antigen Sib(a) has been highly implicated in the pathogenesis 
      of refractoriness to human leukocyte antigen (HLA)-matched platelet transfusions 
      in Japan. We provide evidence that the Sib(a) antigen is located on the 
      glycoprotein (GP) Ib alpha and has a close association with the molecular weight 
      (mol wt) polymorphism of GPIb. In modified antigen-capture ELISA (MACE), 
      anti-Sib(a) antibody reacted only with GPIb/IX held by a murine anti-GPIb/IX 
      monoclonal antibody (MoAb). The reactivity of anti-Sib(a) antibody to 
      Sib(a)-positive (Sib(a+)) platelets was abolished after they were treated with 
      Serratia marcescens protease. Platelets from 50 healthy volunteers were 
      semiquantitatively phenotyped for Sib(a) antigen by MACE and divided into three 
      distinct groups: strongly positive, positive, and negative. They were also 
      analyzed by sodium dodeyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) 
      and periodic acid-silver staining for mol wt polymorphism of GPIb, phenotyped as 
      A, B, C, or D. Without exception, Sib(a+) platelets showed larger phenotypes (A 
      or B). Removal of sialic acid from Sib(a+) platelets did not reduce the binding 
      of anti-Sib(a). Finally, anti-Sib(a) antibody specifically immunoprecipitated A 
      and B phenotypes of GPIb from Sib(a+) platelets. Thus, Sib(a) antigen evidently 
      is located in the region of glycocalicin that is present only on the A and B 
      phenotypes of GPIb.
FAU - Ishida, F
AU  - Ishida F
AD  - Second Department of Internal Medicine, Shinshu University School of Medicine, 
      Nagano, Japan.
FAU - Saji, H
AU  - Saji H
FAU - Maruya, E
AU  - Maruya E
FAU - Furihata, K
AU  - Furihata K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (2b alloantigen, human)
RN  - 0 (Antigens, Human Platelet)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - EC 3.2.1.18 (Neuraminidase)
SB  - IM
MH  - Antigens, Human Platelet/*analysis/genetics
MH  - Blood Platelets/*immunology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - Immunosorbent Techniques
MH  - Molecular Weight
MH  - Neuraminidase/pharmacology
MH  - Pedigree
MH  - Platelet Membrane Glycoproteins/chemistry/genetics/*immunology
MH  - *Polymorphism, Genetic
EDAT- 1991/10/01 00:00
MHDA- 1991/10/01 00:01
CRDT- 1991/10/01 00:00
PHST- 1991/10/01 00:00 [pubmed]
PHST- 1991/10/01 00:01 [medline]
PHST- 1991/10/01 00:00 [entrez]
AID - S0006-4971(20)81088-X [pii]
PST - ppublish
SO  - Blood. 1991 Oct 1;78(7):1722-9.