PMID- 19126762
OWN - NLM
STAT- MEDLINE
DCOM- 20090807
LR  - 20211020
IS  - 0148-6071 (Print)
IS  - 0148-6071 (Linking)
VI  - 33
IP  - 4
DP  - 2009 Jul-Aug
TI  - Starvation-induced proximal gut mucosal atrophy diminished with aging.
PG  - 411-6
LID - 10.1177/0148607108325178 [doi]
AB  - BACKGROUND: Starvation induces small bowel atrophy with increased intestinal 
      epithelial apoptosis and decreased proliferation. The authors examined these 
      parameters after starvation in aged animals. METHODS: Sixty-four 6-week-old and 
      26-month-old C57BL/6 mice were randomly assigned to either an ad libitum fed or 
      fasted group. The small bowel was harvested at 12, 48, and 72 hours following 
      starvation. Proximal gut mucosal height was measured and epithelial cells 
      counted. Apoptosis was identified by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining. Proliferation was 
      determined by immunohistochemical staining for proliferating cell nuclear 
      antigen. Comparison of fed vs fasted and adult vs old groups was done by one-way 
      ANOVA with Tukey's test and unpaired Student's t test. Significance was accepted 
      at P < .05. RESULTS: Aged mice had higher proximal gut weights, mucosal heights, 
      and cell numbers at baseline compared with the adult group (P < .05). The rate of 
      apoptosis was lower in the aged (P < .05), but proliferation was not different 
      between groups before starvation. After starvation, proximal gut wet weight 
      decreased only in adult mice (P < .05). Gut mucosal height and mucosal cell 
      number decreased more in adult than in aged mice (P < .05). This was related to 
      decreased proliferation only in the adult group (P < .05). The fold of epithelial 
      apoptosis that increased was higher in the aged group than in the adult group 
      after starvation (P < .05). CONCLUSIONS: Gut mucosal kinetics change with age and 
      have lower rates of apoptosis and greater mucosal mass; the character of 
      starvation-induced atrophy is diminished with aging.
FAU - Song, Juquan
AU  - Song J
AD  - Shriners Hospitals for Children, Galveston, Texas 77550, USA.
FAU - Wolf, Steven E
AU  - Wolf SE
FAU - Wu, Xiao-Wu
AU  - Wu XW
FAU - Finnerty, Celeste C
AU  - Finnerty CC
FAU - Gauglitz, Gerd G
AU  - Gauglitz GG
FAU - Herndon, David N
AU  - Herndon DN
FAU - Jeschke, Marc G
AU  - Jeschke MG
LA  - eng
GR  - R01 GM-56687/GM/NIGMS NIH HHS/United States
GR  - R01 GM056687/GM/NIGMS NIH HHS/United States
GR  - P50 GM060338/GM/NIGMS NIH HHS/United States
GR  - T32 GM008256/GM/NIGMS NIH HHS/United States
GR  - P50 GM-60338/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090106
PL  - United States
TA  - JPEN J Parenter Enteral Nutr
JT  - JPEN. Journal of parenteral and enteral nutrition
JID - 7804134
RN  - 0 (Proliferating Cell Nuclear Antigen)
SB  - IM
MH  - Age Factors
MH  - Aging/*pathology
MH  - Analysis of Variance
MH  - Animals
MH  - Apoptosis
MH  - Atrophy
MH  - Cell Proliferation
MH  - Disease Models, Animal
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Intestinal Mucosa/*pathology
MH  - Intestine, Small/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Proliferating Cell Nuclear Antigen/analysis
MH  - Starvation/*pathology
PMC - PMC3786366
MID - NIHMS512775
EDAT- 2009/01/08 09:00
MHDA- 2009/08/08 09:00
PMCR- 2013/09/30
CRDT- 2009/01/08 09:00
PHST- 2009/01/08 09:00 [entrez]
PHST- 2009/01/08 09:00 [pubmed]
PHST- 2009/08/08 09:00 [medline]
PHST- 2013/09/30 00:00 [pmc-release]
AID - 0148607108325178 [pii]
AID - 10.1177/0148607108325178 [doi]
PST - ppublish
SO  - JPEN J Parenter Enteral Nutr. 2009 Jul-Aug;33(4):411-6. doi: 
      10.1177/0148607108325178. Epub 2009 Jan 6.