PMID- 19127540
OWN - NLM
STAT- MEDLINE
DCOM- 20090323
LR  - 20181201
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 106
IP  - 3
DP  - 2009 Feb 15
TI  - Role of vascular endothelial growth factor in the communication between human 
      osteoprogenitors and endothelial cells.
PG  - 390-8
LID - 10.1002/jcb.22018 [doi]
AB  - Proper bone remodeling requires an active process of angiogenesis which in turn 
      supplies the necessary growth factors and stem cells. This tissue cooperation 
      suggests a cross-talk between osteoblasts and endothelial cells. This work aims 
      to identify the role of paracrine communication through vascular endothelial 
      growth factor (VEGF) in co-culture between osteoblastic and endothelial cells. 
      Through a well defined direct contact co-culture model between human 
      osteoprogenitors (HOPs) and human umbilical vein endothelial cells (HUVECs), we 
      observed that HUVECs were able to migrate along HOPs, inducing the formation of 
      specific tubular-like structures. VEGF(165) gene expression was detected in the 
      HOPs, was up-regulated in the co-cultured HOPs and both Flt-1 and KDR gene 
      expression increased in co-cultured HUVECs. However, the cell rearrangement 
      observed in co-culture was promoted by a combination of soluble chemoattractive 
      factors and not by VEGF(165) alone. Despite having no observable effect on 
      endothelial cell tubular-like formation, VEGF appeared to have a crucial role in 
      osteoblastic differentiation since the inhibition of its receptors reduced the 
      co-culture-stimulated osteoblastic phenotype. This co-culture system appears to 
      enhance both primary angiogenesis events and osteoblastic differentiation, thus 
      allowing for the development of new strategies in vascularized bone tissue 
      engineering.
FAU - Grellier, Maritie
AU  - Grellier M
AD  - INSERM, U577, Bordeaux and Universite Victor Segalen Bordeaux 2, UMR-S577, 
      F-33076 Bordeaux Cedex, France. maritie.grellier@inserm.fr
FAU - Ferreira-Tojais, Nancy
AU  - Ferreira-Tojais N
FAU - Bourget, Chantal
AU  - Bourget C
FAU - Bareille, Reine
AU  - Bareille R
FAU - Guillemot, Fabien
AU  - Guillemot F
FAU - Amedee, Joelle
AU  - Amedee J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor)
SB  - IM
MH  - *Cell Communication
MH  - *Cell Differentiation
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Endothelial Cells/*cytology/*metabolism
MH  - Enzyme Activation
MH  - Gene Expression Regulation/genetics
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts/*cytology/*metabolism
MH  - RNA, Messenger/genetics
MH  - Receptors, Vascular Endothelial Growth Factor/metabolism
MH  - Umbilical Cord/cytology/metabolism
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
EDAT- 2009/01/08 09:00
MHDA- 2009/03/24 09:00
CRDT- 2009/01/08 09:00
PHST- 2009/01/08 09:00 [entrez]
PHST- 2009/01/08 09:00 [pubmed]
PHST- 2009/03/24 09:00 [medline]
AID - 10.1002/jcb.22018 [doi]
PST - ppublish
SO  - J Cell Biochem. 2009 Feb 15;106(3):390-8. doi: 10.1002/jcb.22018.