PMID- 19128898
OWN - NLM
STAT- MEDLINE
DCOM- 20090804
LR  - 20171116
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 136
IP  - 3-4
DP  - 2009 May 12
TI  - Modulations of phenotype and cytokine expression of porcine bone marrow-derived 
      dendritic cells by porcine reproductive and respiratory syndrome virus.
PG  - 359-65
LID - 10.1016/j.vetmic.2008.11.013 [doi]
AB  - Phenotypic and functional property changes of bone marrow-derived immature 
      dendritic cells (BM-imDCs) after porcine reproductive and respiratory syndrome 
      virus (PRRSV) infection have been detailed in a previous report. A down-regulated 
      expression of MHC I molecules along with an up-regulated expression of CD80/86 
      were observed in BM-imDCs after the exposure to PRRSV. In this study, we further 
      investigate the expression of surface phenotypes of BM-imDCs in relation to their 
      infection status. Exposure of PRRSV to BM-imDCs resulted in a down-regulated 
      expression of MHC I and an up-regulated expression of CD80/86 in infected cells, 
      as demonstrated by significant alterations in both percentage of expressing cells 
      and mean fluorescence intensity (MFI) in PRRSV-positive cells. A significant 
      suppression in MFI of MHC I and an increase in percentage of cells expressing 
      CD80/86 were observed in noninfected bystander cells. We also demonstrated that 
      exposure of BM-imDCs to PRRSV resulted in a significantly increased secretion of 
      IL-1, IL-6, IL-8, IL-10 and IFN-gamma but not IL-12 or TNF-alpha. In addition, 
      the PRRSV infection modulates cytokine expressions of BM-imDCs through their 
      response to microbial pathogen-associated molecular patterns. These results will 
      prove helpful in clarification of the factors that mediate host defense against 
      PRRSV, as well as the possible interaction mechanisms between PRRSV and other 
      microbes in the pathogenesis of PRRSV infection in pigs.
FAU - Peng, Yu-Tang
AU  - Peng YT
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan, ROC.
FAU - Chaung, Hso-Chi
AU  - Chaung HC
FAU - Chang, Hsiu-Luan
AU  - Chang HL
FAU - Chang, Hsueh-Chen
AU  - Chang HC
FAU - Chung, Wen-Bin
AU  - Chung WB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081128
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (B7-1 Antigen)
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Teichoic Acids)
RN  - 56411-57-5 (lipoteichoic acid)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Animals
MH  - B7-1 Antigen/immunology
MH  - Cytokines/biosynthesis/genetics/*immunology
MH  - Dendritic Cells/immunology/*virology
MH  - Flow Cytometry/veterinary
MH  - Histocompatibility Antigens Class I/immunology
MH  - Histocompatibility Antigens Class II/immunology
MH  - Immunophenotyping/veterinary
MH  - Lipopolysaccharides/immunology
MH  - Poly I-C/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Teichoic Acids/immunology
EDAT- 2009/01/09 09:00
MHDA- 2009/08/06 09:00
CRDT- 2009/01/09 09:00
PHST- 2008/07/10 00:00 [received]
PHST- 2008/11/19 00:00 [revised]
PHST- 2008/11/24 00:00 [accepted]
PHST- 2009/01/09 09:00 [entrez]
PHST- 2009/01/09 09:00 [pubmed]
PHST- 2009/08/06 09:00 [medline]
AID - S0378-1135(08)00542-7 [pii]
AID - 10.1016/j.vetmic.2008.11.013 [doi]
PST - ppublish
SO  - Vet Microbiol. 2009 May 12;136(3-4):359-65. doi: 10.1016/j.vetmic.2008.11.013. 
      Epub 2008 Nov 28.