PMID- 19130734
OWN - NLM
STAT- MEDLINE
DCOM- 20090210
LR  - 20191111
IS  - 0722-5091 (Print)
IS  - 0722-5091 (Linking)
VI  - 27
IP  - 6
DP  - 2008 Nov-Dec
TI  - Detecting chromosomal alterations at 1p and 19q by FISH and DNA fragment 
      analysis--a comparative study in human gliomas.
PG  - 378-87
AB  - Histological classification of gliomas is important for treatment and as a 
      prognostic predictor, but classification by histology alone can be a challenge. 
      Molecular genetic investigations, in particular the combined loss of the short 
      arm of chromosome 1 and the long arm of chromosome 19 (LOH1p/19q), has become a 
      significant predictor of outcome in oligodendrogliomas. 1p/19q alterations can be 
      investigated by fluorescence in situ hybridization (FISH), but controversies 
      persist in the interpretation ofresults. Another technique is polymerase chain 
      reaction (PCR) analysis using microsatellites as primers and capillary 
      electrophoresis or southern blot as detection method. The objective of the 
      present study was to compare the accuracy, reliability and feasibility of 
      detecting chromosomal changes at 1p/19q with PCR microsatellite analysis and FISH 
      in glial tumors in the clinical laboratory, where often only small formalin-fixed 
      paraffin-embedded samples are available. Commercial DNA and normal cortex were 
      used for comparison. The material comprised 41 glial tumors including 10 
      oligodendrogliomas (WHO Grades II and III, 5 each), 10 mixed oligoastrocytomas 
      (WHO Grades II and III, 5 each), 10 astrocytomas (WHO Grades II and III, 5 each), 
      and 11 glioblastomas (WHO Grade IV). Our data confirmed a correlation between 
      FISH and LOH fragment analysis in classical oligodendrogliomas and in mixed 
      oligoastrocytomas. Disparity was found among the glioblastomas, where fragment 
      analysis showed 1p/19q loss in three cases, with no changes detected by FISH. The 
      fragment analysis seems reliable and implementable for LOH 1p/19q investigation 
      without patient-related control material.
FAU - Broholm, H
AU  - Broholm H
AD  - Laboratory of Neuropathology, Copenhagen University Hospital, Rigshospitalet, 
      Copenhagen, Denmark. hbroholm@rh.dk
FAU - Born, P W
AU  - Born PW
FAU - Guterbaum, D
AU  - Guterbaum D
FAU - Dyrbye, H
AU  - Dyrbye H
FAU - Laursen, H
AU  - Laursen H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Clin Neuropathol
JT  - Clinical neuropathology
JID - 8214420
SB  - IM
MH  - Brain Neoplasms/genetics/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 19
MH  - DNA Fragmentation
MH  - Feasibility Studies
MH  - Glioma/*genetics/pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity
MH  - Microsatellite Repeats
MH  - *Polymerase Chain Reaction
MH  - Reproducibility of Results
EDAT- 2009/01/10 09:00
MHDA- 2009/02/12 09:00
CRDT- 2009/01/10 09:00
PHST- 2009/01/10 09:00 [entrez]
PHST- 2009/01/10 09:00 [pubmed]
PHST- 2009/02/12 09:00 [medline]
AID - 5060 [pii]
AID - 10.5414/npp27378 [doi]
PST - ppublish
SO  - Clin Neuropathol. 2008 Nov-Dec;27(6):378-87. doi: 10.5414/npp27378.