PMID- 19131259
OWN - NLM
STAT- MEDLINE
DCOM- 20100303
LR  - 20181201
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 183
IP  - 2
DP  - 2010 Feb
TI  - Simultaneous detection of porcine circovirus type 2, classical swine fever virus, 
      porcine parvovirus and porcine reproductive and respiratory syndrome virus in 
      pigs by multiplex polymerase chain reaction.
PG  - 172-5
LID - 10.1016/j.tvjl.2008.11.016 [doi]
AB  - A multiplex polymerase chain reaction (PCR) was designed for the simultaneous 
      detection of four viruses involved in reproductive and respiratory failure in 
      pigs: porcine circovirus type 2 (PCV-2), porcine parvovirus (PPV), classical 
      swine fever virus (CSFV) and porcine reproductive and respiratory syndrome virus 
      (PRRSV). Each of the four pairs of oligonucleotide primers exclusively amplified 
      the targeted fragment of the specific viruses. The sensitivity of the multiplex 
      PCR using purified plasmid constructs containing the specific viral target 
      fragments was 2.58x10(7), 2.64x10(5), 2.66x10(7) and 2.73x10(5) copies for PRRSV, 
      PCV-2, CSFV and PPV, respectively. Using the multiplex PCR, co-infections with 
      these four viruses were identified in 26/76 (34.2%) piglets born from sows with 
      reproductive failure in China. This method is a rapid, sensitive and 
      cost-effective diagnostic tool for the routine surveillance of viral infections 
      in pigs.
CI  - Copyright (c) 2008 Elsevier Ltd. All rights reserved.
FAU - Jiang, Yonghou
AU  - Jiang Y
AD  - College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China. 
      yonghoujiang@zstu.edu.cn
FAU - Shang, Hanwu
AU  - Shang H
FAU - Xu, Hui
AU  - Xu H
FAU - Zhu, Liangjun
AU  - Zhu L
FAU - Chen, Weijie
AU  - Chen W
FAU - Zhao, Lingyan
AU  - Zhao L
FAU - Fang, Li
AU  - Fang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090107
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Circoviridae Infections/diagnosis/veterinary
MH  - Circovirus/genetics/isolation & purification
MH  - Classical Swine Fever/diagnosis
MH  - Classical Swine Fever Virus/genetics/isolation & purification
MH  - DNA Primers/chemistry
MH  - DNA, Viral/analysis
MH  - Parvoviridae Infections/diagnosis/veterinary
MH  - Parvovirus, Porcine/genetics/isolation & purification
MH  - Polymerase Chain Reaction/*veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/genetics/isolation & 
      purification
MH  - RNA, Viral/analysis
MH  - Sensitivity and Specificity
MH  - Sentinel Surveillance/veterinary
MH  - Swine
MH  - Swine Diseases/*diagnosis/virology
MH  - Virus Diseases/diagnosis/*veterinary
MH  - Viruses/genetics/*isolation & purification
EDAT- 2009/01/10 09:00
MHDA- 2010/03/04 06:00
CRDT- 2009/01/10 09:00
PHST- 2008/08/05 00:00 [received]
PHST- 2008/11/04 00:00 [revised]
PHST- 2008/11/28 00:00 [accepted]
PHST- 2009/01/10 09:00 [entrez]
PHST- 2009/01/10 09:00 [pubmed]
PHST- 2010/03/04 06:00 [medline]
AID - S1090-0233(08)00415-2 [pii]
AID - 10.1016/j.tvjl.2008.11.016 [doi]
PST - ppublish
SO  - Vet J. 2010 Feb;183(2):172-5. doi: 10.1016/j.tvjl.2008.11.016. Epub 2009 Jan 7.