PMID- 19131437 OWN - NLM STAT- MEDLINE DCOM- 20091021 LR - 20090807 IS - 1460-2199 (Electronic) IS - 1047-3211 (Linking) VI - 19 IP - 9 DP - 2009 Sep TI - Excitatory GABAergic activation of cortical dividing glial cells. PG - 2181-95 LID - 10.1093/cercor/bhn238 [doi] AB - Adult neocortex contains dividing satellite glia population even though their characteristics and functions have still remained unknown. Nestin(+)/NG2(+) cells as major fraction of dividing glial cells express bicuculline-sensitive gamma-aminobutyric acid A (GABA(A)) receptors and receive GABAergic inputs. Due to their high [Cl(-)](i), GABAergic activation depolarized the cells and then induced Ca(2+) influx into them. To assess an effect of this GABAergic excitation, we looked for the expression of neurotrophic factors. Among them, we detected the expression of brain-derived neurotrophic factor (BDNF) on the cells. The level of BDNF expression was elevated after cortical ischemia, and this elevation was blocked by bumetanide, an inhibitor for NKCC1 that blocks the GABAergic depolarization. Furthermore, performing a modified adhesive removal test, we observed that the treatment of bumetanide significantly attenuated the recovery in somatosensory dysfunction. Our results may shed a light on satellite glia population in the cortex and imply their roles in the functional recovery after ischemic injuries. FAU - Tanaka, Yuichi AU - Tanaka Y AD - Department of Integrated Biosciences, University of Tokyo, Kashiwa, Chiba 277-8562, Japan. FAU - Tozuka, Yusuke AU - Tozuka Y FAU - Takata, Tetsuo AU - Takata T FAU - Shimazu, Naoyuki AU - Shimazu N FAU - Matsumura, Naoto AU - Matsumura N FAU - Ohta, Aya AU - Ohta A FAU - Hisatsune, Tatsuhiro AU - Hisatsune T LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090108 PL - United States TA - Cereb Cortex JT - Cerebral cortex (New York, N.Y. : 1991) JID - 9110718 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 56-12-2 (gamma-Aminobutyric Acid) SB - IM MH - Action Potentials/*physiology MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cerebral Cortex/*physiology MH - Excitatory Postsynaptic Potentials/*physiology MH - Male MH - Neuroglia MH - Rats MH - Rats, Sprague-Dawley MH - gamma-Aminobutyric Acid/*metabolism EDAT- 2009/01/10 09:00 MHDA- 2009/10/22 06:00 CRDT- 2009/01/10 09:00 PHST- 2009/01/10 09:00 [entrez] PHST- 2009/01/10 09:00 [pubmed] PHST- 2009/10/22 06:00 [medline] AID - bhn238 [pii] AID - 10.1093/cercor/bhn238 [doi] PST - ppublish SO - Cereb Cortex. 2009 Sep;19(9):2181-95. doi: 10.1093/cercor/bhn238. Epub 2009 Jan 8.