PMID- 19132369
OWN - NLM
STAT- MEDLINE
DCOM- 20090511
LR  - 20131121
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 83
IP  - 1
DP  - 2009 May
TI  - Characterization of beta-1,3-galactosyl-N-acetylhexosamine phosphorylase from 
      Propionibacterium acnes.
PG  - 109-15
LID - 10.1007/s00253-008-1838-y [doi]
AB  - Homologs of the beta-1,3-galactosyl-N-acetylhexosamine phosphorylase (GalHexNAcP) 
      gene (gnpA) were cloned from the genomic DNA of Propionibacterium acnes JCM6425 
      and P. acnes JCM6473, showing 99.9% and 97.9% nucleotide sequence identity, 
      respectively, with the ppa0083 gene from the genome-sequenced P. acnes KPA171202. 
      No gnpA gene was detected in the genomic DNA of type strain P. acnes ATCC25746. 
      The recombinant enzyme from P. acnes JCM6425 (GnpA) showed approximately 70 times 
      higher specific activity of phosphorolysis on galacto-N-biose 
      (Galbeta1-->3GalNAc, GNB) than that on lacto-N-biose I (Galbeta1-->3GlcNAc). K 
      (m) value for GnpA on GNB was high, but GnpA did not exhibit activity on any 
      derivatives of GNB examined. These results indicate that GnpA is GalHexNAcP which 
      should be classified as galacto-N-biose phosphorylase. The large k (cat) value of 
      GnpA on GalNAc suggests that GnpA would be a useful catalyst for the synthesis of 
      GNB.
FAU - Nakajima, Masahiro
AU  - Nakajima M
AD  - National Food Research Institute, National Agriculture and Food Research 
      Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
FAU - Nishimoto, Mamoru
AU  - Nishimoto M
FAU - Kitaoka, Motomitsu
AU  - Kitaoka M
LA  - eng
SI  - GENBANK/AB468065
SI  - GENBANK/AB468066
PT  - Journal Article
DEP - 20090109
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (DNA, Bacterial)
RN  - 0 (Disaccharides)
RN  - 5077-31-6 (6-O-galactopyranosylgalactose)
RN  - 50787-09-2 (galactosyl-1,3-N-acetylglucosamine)
RN  - EC 2.4.1.- (Galactosyltransferases)
RN  - EC 2.4.1.- (beta-1,3-galactosyl-N-acetylhexosamine phosphorylase)
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine/analogs & derivatives/metabolism
MH  - Cloning, Molecular
MH  - DNA, Bacterial/chemistry/genetics
MH  - Disaccharides/metabolism
MH  - Enzyme Stability
MH  - Galactosyltransferases/*genetics/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Propionibacterium acnes/*enzymology/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Substrate Specificity
MH  - Temperature
EDAT- 2009/01/10 09:00
MHDA- 2009/05/12 09:00
CRDT- 2009/01/10 09:00
PHST- 2008/11/06 00:00 [received]
PHST- 2008/12/21 00:00 [accepted]
PHST- 2008/12/12 00:00 [revised]
PHST- 2009/01/10 09:00 [entrez]
PHST- 2009/01/10 09:00 [pubmed]
PHST- 2009/05/12 09:00 [medline]
AID - 10.1007/s00253-008-1838-y [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2009 May;83(1):109-15. doi: 10.1007/s00253-008-1838-y. 
      Epub 2009 Jan 9.