PMID- 19132682
OWN - NLM
STAT- MEDLINE
DCOM- 20090505
LR  - 20220309
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 9
IP  - 3
DP  - 2009 Feb
TI  - Mass spectrometric and computational analysis of cytokine-induced alterations in 
      the astrocyte secretome.
PG  - 768-82
LID - 10.1002/pmic.200800385 [doi]
AB  - The roles of astrocytes in the CNS have been expanding beyond the long held view 
      of providing passive, supportive functions. Recent evidence has identified roles 
      in neuronal development, extracellular matrix maintenance, and response to 
      inflammatory challenges. Therefore, insights into astrocyte secretion are 
      critically important for understanding physiological responses and pathological 
      mechanisms in CNS diseases. Primary astrocyte cultures were treated with 
      inflammatory cytokines for either a short (1 day) or sustained (7 days) exposure. 
      Increased interleukin-6 secretion, nitric oxide production, cyclooxygenase-2 
      activation, and nerve growth factor (NGF) secretion confirmed the astrocytic 
      response to cytokine treatment. MS/MS analysis, computational prediction 
      algorithms, and functional classification were used to compare the astrocyte 
      protein secretome from control and cytokine-exposed cultures. In total, 169 
      secreted proteins were identified, including both classically and 
      nonconventionally secreted proteins that comprised components of the 
      extracellular matrix and enzymes involved in processing of glycoproteins and 
      glycosaminoglycans. Twelve proteins were detected exclusively in the secretome 
      from cytokine-treated astrocytes, including matrix metalloproteinase-3 (MMP-3) 
      and members of the chemokine ligand family. This compilation of secreted proteins 
      provides a framework for identifying factors that influence the biochemical 
      environment of the nervous system, regulate development, construct extracellular 
      matrices, and coordinate the nervous system response to inflammation.
FAU - Keene, Sarah Dunn
AU  - Keene SD
AD  - Stokes Research Institute and Department of Pediatrics, Children's Hospital of 
      Philadelphia, Philadelphia, PA 19104-4318, USA.
FAU - Greco, Todd M
AU  - Greco TM
FAU - Parastatidis, Ioannis
AU  - Parastatidis I
FAU - Lee, Seon-Hwa
AU  - Lee SH
FAU - Hughes, Ethan G
AU  - Hughes EG
FAU - Balice-Gordon, Rita J
AU  - Balice-Gordon RJ
FAU - Speicher, David W
AU  - Speicher DW
FAU - Ischiropoulos, Harry
AU  - Ischiropoulos H
LA  - eng
GR  - AG13966/AG/NIA NIH HHS/United States
GR  - R01 AG013966-11/AG/NIA NIH HHS/United States
GR  - R21 MH084237/MH/NIMH NIH HHS/United States
GR  - R01 AG013966/AG/NIA NIH HHS/United States
GR  - P30 ES013508/ES/NIEHS NIH HHS/United States
GR  - ES013508/ES/NIEHS NIH HHS/United States
GR  - R21 MH084237-01A2/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Astrocytes/*drug effects/*metabolism
MH  - Cells, Cultured
MH  - Cytokines/*pharmacology
MH  - Mice
MH  - Proteomics/methods
MH  - Tandem Mass Spectrometry/*methods
PMC - PMC2667946
MID - NIHMS99991
EDAT- 2009/01/10 09:00
MHDA- 2009/05/06 09:00
PMCR- 2009/04/12
CRDT- 2009/01/10 09:00
PHST- 2009/01/10 09:00 [entrez]
PHST- 2009/01/10 09:00 [pubmed]
PHST- 2009/05/06 09:00 [medline]
PHST- 2009/04/12 00:00 [pmc-release]
AID - 10.1002/pmic.200800385 [doi]
PST - ppublish
SO  - Proteomics. 2009 Feb;9(3):768-82. doi: 10.1002/pmic.200800385.