PMID- 19132893
OWN - NLM
STAT- MEDLINE
DCOM- 20090916
LR  - 20131121
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 15
IP  - 7
DP  - 2009 Jul
TI  - In vivo osteogenic differentiation of human adipose-derived stem cells in an 
      injectable in situ-forming gel scaffold.
PG  - 1821-32
LID - 10.1089/ten.tea.2008.0386 [doi]
AB  - The sol-to-gel transition occurring at around body temperature makes the MPEG-PCL 
      diblock copolymer an ideal candidate material for use as an injectable in 
      situ-forming gel containing human adipose tissue-derived stem cells (hADSCs). The 
      sol can be prepared at room temperature, and the gel forms at body temperature. 
      Solutions of the copolymer containing hADSCs and osteogenic factors injected into 
      rats formed gel scaffolds at the injection sites. The gels thus formed showed the 
      interconnective pore structure required to support growth, proliferation, and 
      differentiation of hADSCs. Bromodeoxyuridine-labeled hADSCs were confirmed to be 
      present in gels formed in vivo. Bone formation was observed only in gel implants 
      containing both hADSCs and osteogenic factors. Subcutaneous implantation of the 
      in situ-forming gel scaffold demonstrated that hADSCs embedded in the gel 
      stimulated much lower host tissue responses than did the gel alone, probably 
      because of the unique immunomodulatory properties of hADSCs. In conclusion, our 
      data on hADSCs embedded in an in situ gel scaffold suggest that this formulation 
      may provide numerous benefits as a noninvasive alternative for tissue-engineered 
      bone formation.
FAU - Ahn, Hyun Hee
AU  - Ahn HH
AD  - Fusion Biotechnology Research Center, Korea Research Institute of Chemical 
      Technology, Daejeon, Korea.
FAU - Kim, Kyung Sook
AU  - Kim KS
FAU - Lee, Jung Hwa
AU  - Lee JH
FAU - Lee, Ju Young
AU  - Lee JY
FAU - Kim, Byung Soo
AU  - Kim BS
FAU - Lee, Il Woo
AU  - Lee IW
FAU - Chun, Heung Jae
AU  - Chun HJ
FAU - Kim, Jae Ho
AU  - Kim JH
FAU - Lee, Hai Bang
AU  - Lee HB
FAU - Kim, Moon Suk
AU  - Kim MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Gels)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Adipocytes/*cytology
MH  - Animals
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Adhesion
MH  - *Cell Differentiation
MH  - Cell Survival
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Gels
MH  - Humans
MH  - Inflammation
MH  - Injections
MH  - Injections, Subcutaneous
MH  - *Osteogenesis
MH  - Rats
MH  - Rats, Inbred F344
MH  - Staining and Labeling
MH  - Stem Cells/*cytology/ultrastructure
MH  - Temperature
MH  - *Tissue Scaffolds
MH  - Viscosity
EDAT- 2009/01/10 09:00
MHDA- 2009/09/17 06:00
CRDT- 2009/01/10 09:00
PHST- 2009/01/10 09:00 [entrez]
PHST- 2009/01/10 09:00 [pubmed]
PHST- 2009/09/17 06:00 [medline]
AID - 10.1089/ten.tea.2008.0386 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2009 Jul;15(7):1821-32. doi: 10.1089/ten.tea.2008.0386.