PMID- 19133265
OWN - NLM
STAT- MEDLINE
DCOM- 20100317
LR  - 20211020
IS  - 1879-0135 (Electronic)
IS  - 0020-7519 (Print)
IS  - 0020-7519 (Linking)
VI  - 39
IP  - 6
DP  - 2009 May
TI  - Revised karyotyping and gene mapping of the Biomphalaria glabrata embryonic (Bge) 
      cell line.
PG  - 675-81
LID - 10.1016/j.ijpara.2008.11.011 [doi]
AB  - The fresh water snail Biomphalaria glabrata (2n=36) belongs to the taxonomic 
      class Gastropoda (family Planorbidae) and is integral to the spread of the human 
      parasitic disease schistosomiasis. The importance of this mollusc is such that it 
      has been selected as a model molluscan organism for whole genome sequencing. In 
      order to understand the structure and organisation of the B. glabrata's genome it 
      is important that gene mapping studies are established. Thus, we have studied the 
      genomes of two B. glabrata embryonic (Bge) cell line isolates 1 and 2 grown in 
      separate laboratories, but both derived from Eder L. Hansen's original culture 
      from the 1970s. This cell line continues to be an important tool and model system 
      for schistosomiasis and B. glabrata. Using these cell line isolates, we have 
      investigated the genome content and established a revised karyotype based on 
      chromosome size and centromere position for these cells. Unlike the original 
      karyotype (2n=36) established for the cell line, our investigations now show the 
      existence of extensive aneuploidy in both cell line isolates to the extent that 
      the total complement of chromosomes in both greatly exceeds the original cell 
      line's diploid number of 36 chromosomes. The isolates, designated Bge 1 and 2, 
      had modal chromosome complements of 64 and 67, respectively (calculated from 50 
      metaphases). We found that the aneuploidy was most pronounced, for both isolates, 
      amongst chromosomes of medium metacentric morphology. We also report, to our 
      knowledge for the first time using Bge cells, the mapping of single-copy genes 
      peroxiredoxin (BgPrx4) and P-element induced wimpy testis (piwi) onto Bge 
      chromosomes. These B. glabrata genes were mapped onto pairs of homologous 
      chromosomes using fluorescence in situ hybridization (FISH). Thus, we have now 
      established a FISH mapping technique that can eventually be utilized for physical 
      mapping of the snail genome.
FAU - Odoemelam, Edwin
AU  - Odoemelam E
AD  - Laboratory of Nuclear and Genomic Health, Centre for Cell and Chromosome Biology, 
      Biosciences, School of Health Sciences and Social Care, Brunel University, West 
      London UB8 3PH, UK.
FAU - Raghavan, Nithya
AU  - Raghavan N
FAU - Miller, Andre
AU  - Miller A
FAU - Bridger, Joanna M
AU  - Bridger JM
FAU - Knight, Matty
AU  - Knight M
LA  - eng
GR  - R01 AI063480/AI/NIAID NIH HHS/United States
GR  - R01 AI063480-01A1/AI/NIAID NIH HHS/United States
GR  - AI63480/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20081224
PL  - England
TA  - Int J Parasitol
JT  - International journal for parasitology
JID - 0314024
SB  - IM
MH  - Animals
MH  - Biomphalaria/*genetics
MH  - Chromosome Mapping
MH  - *Chromosomes
MH  - *Genome
MH  - Karyotyping
PMC - PMC2656398
MID - NIHMS84003
EDAT- 2009/01/10 09:00
MHDA- 2010/03/18 06:00
PMCR- 2010/05/01
CRDT- 2009/01/10 09:00
PHST- 2008/09/23 00:00 [received]
PHST- 2008/11/03 00:00 [revised]
PHST- 2008/11/19 00:00 [accepted]
PHST- 2009/01/10 09:00 [entrez]
PHST- 2009/01/10 09:00 [pubmed]
PHST- 2010/03/18 06:00 [medline]
PHST- 2010/05/01 00:00 [pmc-release]
AID - S0020-7519(08)00476-1 [pii]
AID - 10.1016/j.ijpara.2008.11.011 [doi]
PST - ppublish
SO  - Int J Parasitol. 2009 May;39(6):675-81. doi: 10.1016/j.ijpara.2008.11.011. Epub 
      2008 Dec 24.