PMID- 19136142
OWN - NLM
STAT- MEDLINE
DCOM- 20090507
LR  - 20181201
IS  - 0048-9697 (Print)
IS  - 0048-9697 (Linking)
VI  - 407
IP  - 5
DP  - 2009 Feb 15
TI  - Structural and physiological responses to ozone in Manna ash (Fraxinus ornus L.) 
      leaves of seedlings and mature trees under controlled and ambient conditions.
PG  - 1631-43
LID - 10.1016/j.scitotenv.2008.11.061 [doi]
AB  - Leaf-level microscopical symptom structure and physiological responses were 
      investigated in seedlings experimentally exposed to ozone (O3) in indoor chambers 
      (150 ppb, 8 h d(-1)/7 weeks), and field trees of Manna ash (Fraxinus ornus) 
      exposed to ambient O3 (max 93 ppb/one growing season). Ozone-induced leaf injury, 
      including leaf reddening and stippling, was observed in both seedlings and mature 
      trees, but the morphology of injury in the stipples differed, being 
      hypersensitive-like (HR-like) in the chamber seedlings and accelerated cell 
      senescence (ACS) in the field trees. In both exposure conditions, the main 
      structural impact of O3 was on the mesophyll and especially the upper 
      assimilating cell layers. The main physiological impact was on carbon 
      assimilation and on stomatal sluggishness. These effects were not due to stomatal 
      structural injury and were more severe in juvenile compared to mature trees 
      because of environmental (water availability, light) and constitutional (gas 
      exchange capacity) factors and differences in the cell physiology processes 
      (HR-like vs. ACS) triggered by ozone stress. Given the plasticity of plant 
      responses to ozone stress, dose/response relationships for tree seedlings in the 
      indoor chambers cannot be extrapolated to mature trees unless ambient conditions 
      are closely simulated.
FAU - Paoletti, Elena
AU  - Paoletti E
AD  - Institute Plant Protection, National Council of Research, Via Madonna del Piano 
      10, I-50019 Sesto Fiorentino, Florence, Italy. e.paoletti@ipp.cnr.it
FAU - Contran, Nicla
AU  - Contran N
FAU - Bernasconi, Petra
AU  - Bernasconi P
FAU - Gunthardt-Goerg, Madeleine S
AU  - Gunthardt-Goerg MS
FAU - Vollenweider, Pierre
AU  - Vollenweider P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090110
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Air Pollutants)
RN  - 1406-65-1 (Chlorophyll)
RN  - 66H7ZZK23N (Ozone)
RN  - YF5Q9EJC8Y (Chlorophyll A)
SB  - IM
EIN - Sci Total Environ. 2010 Mar 15;408(8):2013
CRI - Sci Total Environ. 2010 Mar 15;408(8):2014-24. PMID: 20238440
MH  - Air Pollutants/*toxicity
MH  - Chlorophyll/analysis/metabolism
MH  - Chlorophyll A
MH  - Fraxinus/*drug effects/metabolism/*physiology/ultrastructure
MH  - Microscopy, Fluorescence
MH  - Ozone/*toxicity
MH  - Photosynthesis/drug effects
MH  - Plant Leaves/drug effects/metabolism/physiology/ultrastructure
MH  - Plant Stomata/drug effects/metabolism/physiology/ultrastructure
MH  - Random Allocation
MH  - Trees/drug effects/physiology
EDAT- 2009/01/13 09:00
MHDA- 2009/05/08 09:00
CRDT- 2009/01/13 09:00
PHST- 2008/09/24 00:00 [received]
PHST- 2008/11/24 00:00 [revised]
PHST- 2008/11/24 00:00 [accepted]
PHST- 2009/01/13 09:00 [entrez]
PHST- 2009/01/13 09:00 [pubmed]
PHST- 2009/05/08 09:00 [medline]
AID - S0048-9697(08)01217-5 [pii]
AID - 10.1016/j.scitotenv.2008.11.061 [doi]
PST - ppublish
SO  - Sci Total Environ. 2009 Feb 15;407(5):1631-43. doi: 
      10.1016/j.scitotenv.2008.11.061. Epub 2009 Jan 10.