PMID- 19136885
OWN - NLM
STAT- MEDLINE
DCOM- 20090130
LR  - 20171116
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 87
IP  - 1
DP  - 2009 Jan 15
TI  - Dimerization of soluble HLA-G by IgG-Fc fragment augments ILT2-mediated 
      inhibition of T-cell alloresponse.
PG  - 8-15
LID - 10.1097/TP.0b013e31818b6141 [doi]
AB  - BACKGROUND: Human leukocyte antigen (HLA)-G, a nonclassical HLA class I molecule, 
      induces a wide range of tolerogenic immunological effects by means of interaction 
      with its inhibitory receptors. However, recent studies show that HLA-G dimer 
      formation is essential to bind to its receptors and exhibit its effects. METHODS: 
      In this study, a soluble divalent HLA-G/IgG molecule (sHLA-G dimer) was 
      constructed. Its inhibitory effect on T-cell alloresponse was studied with mixed 
      lymphocyte reaction in vitro, which was set up by mixing inactivated T1 cells 
      with HLA-mismatched peripheral blood lymphocytes in the presence or absence of 
      the sHLA-G dimer. RESULTS: The results show that sHLA-G dimer inhibits T-cell 
      alloresponse by reducing proliferation of both CD4+ and CD8+ T cells and 
      suppressing generation of alloreactive cytotoxic T lymphocytes at nanomole 
      concentration. The inhibition of the sHLA-G dimer is observed to be more 
      effective than that of sHLA-G monomer. Our results also indicate that sHLA-G 
      dimer up-regulates inhibitory receptor ILT2 on alloreactive CD8+ T cells, which 
      contributes to the significant inhibition on T-cell alloresponse. CONCLUSION: The 
      sHLA-G dimer formed by IgG-Fc fragment shows more inhibitory effects on 
      alloreactive T cells, which may have implications for allotransplantation.
FAU - Zhong, Maohua
AU  - Zhong M
AD  - Department of Immunology, Tongji Medical College, Huazhong University of Science 
      and Technology, Wuhan, China.
FAU - Weng, Xiufang
AU  - Weng X
FAU - Liang, Zhihui
AU  - Liang Z
FAU - Lu, Shengjun
AU  - Lu S
FAU - Li, Jianan
AU  - Li J
FAU - Chen, Xueling
AU  - Chen X
FAU - Li, Qing
AU  - Li Q
FAU - Sun, Wei
AU  - Sun W
FAU - Song, Yinhong
AU  - Song Y
FAU - Shen, Guanxin
AU  - Shen G
FAU - Wu, Xiongwen
AU  - Wu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antigens, CD)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 0 (LILRB1 protein, human)
RN  - 0 (Leukocyte Immunoglobulin-like Receptor B1)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Antigens, CD/*immunology
MH  - Autoimmunity/*immunology
MH  - Cell Line
MH  - Cell Proliferation
MH  - HLA Antigens/*immunology/metabolism
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*immunology/metabolism
MH  - Humans
MH  - Immunoglobulin Fc Fragments/*immunology
MH  - Immunoglobulin G/*immunology
MH  - Leukocyte Immunoglobulin-like Receptor B1
MH  - Protein Multimerization/*immunology
MH  - Receptors, Immunologic/*immunology
MH  - Solubility
MH  - T-Lymphocytes/cytology/*immunology
MH  - Up-Regulation/immunology
EDAT- 2009/01/13 09:00
MHDA- 2009/01/31 09:00
CRDT- 2009/01/13 09:00
PHST- 2009/01/13 09:00 [entrez]
PHST- 2009/01/13 09:00 [pubmed]
PHST- 2009/01/31 09:00 [medline]
AID - 00007890-200901150-00003 [pii]
AID - 10.1097/TP.0b013e31818b6141 [doi]
PST - ppublish
SO  - Transplantation. 2009 Jan 15;87(1):8-15. doi: 10.1097/TP.0b013e31818b6141.