PMID- 19137082
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 2005-3711 (Print)
IS  - 1598-7876 (Electronic)
IS  - 1225-8245 (Linking)
VI  - 44
IP  - 6
DP  - 2008 Dec
TI  - Effect of single growth factor and growth factor combinations on differentiation 
      of neural stem cells.
PG  - 375-81
LID - 10.3340/jkns.2008.44.6.375 [doi]
AB  - OBJECTIVE: The effects on neural proliferation and differentiation of neural stem 
      cells (NSC) of basic fibroblast growth factor-2 (bFGF), insulin growth factor-I 
      (IGF-I), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) 
      were assessed. Also, following combinations of various factors were investigated 
      : bFGF+IGF-I, bFGF+BDNF, bFGF+NGF, IGF-I+BDNF, IGF-I+NGF, and BDNF+NGF. METHODS: 
      Isolated NSC of Fisher 344 rats were cultured with individual growth factors, 
      combinations of factors, and no growth factor (control) for 14 days. A proportion 
      of neurons was analyzed using beta-tubulin III and NeuN as neural markers. 
      RESULTS: Neural differentiations in the presence of individual growth factors for 
      beta-tubulin III-positive cells were : BDNF, 35.3%; IGF-I, 30.9%; bFGF, 18.1%; 
      and NGF, 15.1%, and for NeuN-positive cells was : BDNF, 34.3%; bFGF, 32.2%; 
      IGF-1, 26.6%; and NGF, 24.9%. However, neural differentiations in the absence of 
      growth factor was only 2.6% for beta-tubulin III and 3.1% for NeuN. For 
      beta-tubulin III-positive cells, neural differentiations were evident for the 
      growth factor combinations as follows : bFGF+IGF-I, 73.1%; bFGF+NGF, 65.4%; 
      bFGF+BDNF, 58.7%; BDNF+IGF-I, 52.2%; NGF+IGF-I, 40.6%; and BDNF+NGF, 40.0%. For 
      NeuN-positive cells : bFGF+IGF-I, 81.9%; bFGF+NGF, 63.5%; bFGF+BDNF, 62.8%; 
      NGF+IGF-I, 62.3%; BDNF+NGF, 56.3%; and BDNF+IGF-I, 46.0%. Significant differences 
      in neural differentiation were evident for single growth factor and combination 
      of growth factors respectively (p<0.05). CONCLUSION: Combinations of growth 
      factors have an additive effect on neural differentiation. The most prominent 
      neural differentiation results from growth factor combinations involving bFGF and 
      IGF-I. These findings suggest that the combination of a mitogenic action of bFGF 
      and postmitotic differentiation action of IGF-I synergistically affects neural 
      proliferation and NSC differentiation.
FAU - Choi, Kyung-Chul
AU  - Choi KC
AD  - Department of Neurosurgery, Uijeongbu St. Mary's Hospital, The Catholic 
      University of Korea College of Medicine, Uijeongbu, Korea.
FAU - Yoo, Do-Sung
AU  - Yoo DS
FAU - Cho, Kyung-Sock
AU  - Cho KS
FAU - Huh, Pil-Woo
AU  - Huh PW
FAU - Kim, Dal-Soo
AU  - Kim DS
FAU - Park, Chun-Kun
AU  - Park CK
LA  - eng
PT  - Journal Article
DEP - 20081231
PL  - Korea (South)
TA  - J Korean Neurosurg Soc
JT  - Journal of Korean Neurosurgical Society
JID - 101467054
PMC - PMC2615141
OTO - NOTNLM
OT  - Combination of growth factors
OT  - Neuron
OT  - Single growth factor
OT  - Synergistic effect
EDAT- 2009/01/13 09:00
MHDA- 2009/01/13 09:01
PMCR- 2008/12/01
CRDT- 2009/01/13 09:00
PHST- 2008/08/13 00:00 [received]
PHST- 2008/11/28 00:00 [accepted]
PHST- 2009/01/13 09:00 [entrez]
PHST- 2009/01/13 09:00 [pubmed]
PHST- 2009/01/13 09:01 [medline]
PHST- 2008/12/01 00:00 [pmc-release]
AID - 10.3340/jkns.2008.44.6.375 [doi]
PST - ppublish
SO  - J Korean Neurosurg Soc. 2008 Dec;44(6):375-81. doi: 10.3340/jkns.2008.44.6.375. 
      Epub 2008 Dec 31.