PMID- 19137548
OWN - NLM
STAT- MEDLINE
DCOM- 20090505
LR  - 20220408
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 9
IP  - 3
DP  - 2009 Feb
TI  - Yeast protein expression profile during acetic acid-induced apoptosis indicates 
      causal involvement of the TOR pathway.
PG  - 720-32
LID - 10.1002/pmic.200700816 [doi]
AB  - Although acetic acid has been shown to induce apoptosis in yeast, the exact 
      apoptotic mechanisms remain unknown. Here, we studied the effects of acetic acid 
      treatment on yeast cells by 2-DE, revealing alterations in the levels of proteins 
      directly or indirectly linked with the target of rapamycin (TOR) pathway: 
      amino-acid biosynthesis, transcription/translation machinery, carbohydrate 
      metabolism, nucleotide biosynthesis, stress response, protein turnover and cell 
      cycle. The increased levels of proteins involved in amino-acid biosynthesis 
      presented a counteracting response to a severe intracellular amino-acid 
      starvation induced by acetic acid. Deletion of GCN4 and GCN2 encoding key players 
      of general amino-acid control (GAAC) system caused a higher resistance to acetic 
      acid indicating an involvement of Gcn4p/Gcn2p in the apoptotic signaling. 
      Involvement of the TOR pathway in acetic acid-induced apoptosis was also 
      reflected by the higher survival rates associated to a terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL)-negative phenotype and lower 
      reactive oxygen species levels of Deltator1 cells. In addition, deletion mutants 
      for several downstream mediators of the TOR pathway revealed that apoptotic 
      signaling involves the phosphatases Pph21p and Pph22p but not Sit4p. Altogether, 
      our results indicate that GAAC and TOR pathways (Tor1p) are involved in the 
      signaling of acetic acid-induced apoptosis.
FAU - Almeida, Bruno
AU  - Almeida B
AD  - Life and Health Sciences Research Institute (ICVS), School of Health Sciences, 
      University of Minho, Braga, Portugal.
FAU - Ohlmeier, Steffen
AU  - Ohlmeier S
FAU - Almeida, Agostinho J
AU  - Almeida AJ
FAU - Madeo, Frank
AU  - Madeo F
FAU - Leao, Cecilia
AU  - Leao C
FAU - Rodrigues, Fernando
AU  - Rodrigues F
FAU - Ludovico, Paula
AU  - Ludovico P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (target of rapamycin protein, S cerevisiae)
RN  - Q40Q9N063P (Acetic Acid)
SB  - IM
MH  - Acetic Acid/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Blotting, Western
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Gene Expression Regulation, Fungal/drug effects
MH  - Mass Spectrometry
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Saccharomyces cerevisiae/drug effects/*metabolism
MH  - Saccharomyces cerevisiae Proteins/*metabolism
MH  - Signal Transduction/drug effects
EDAT- 2009/01/13 09:00
MHDA- 2009/05/06 09:00
CRDT- 2009/01/13 09:00
PHST- 2009/01/13 09:00 [entrez]
PHST- 2009/01/13 09:00 [pubmed]
PHST- 2009/05/06 09:00 [medline]
AID - 10.1002/pmic.200700816 [doi]
PST - ppublish
SO  - Proteomics. 2009 Feb;9(3):720-32. doi: 10.1002/pmic.200700816.