PMID- 19138116
OWN - NLM
STAT- MEDLINE
DCOM- 20090302
LR  - 20211020
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 57
IP  - 3
DP  - 2009 Feb 11
TI  - Quantitative and fingerprint analyses of Chinese sweet tea plant ( Rubus 
      suavissimus S. Lee).
PG  - 1076-83
LID - 10.1021/jf8029397 [doi]
AB  - The quality of botanical food is increasingly assessed by the content of multiple 
      bioactive compounds. This study reports, for the first time, an HPLC 
      fingerprinting method for the quality evaluation of Rubus suavissimus leaves 
      possessing multiple bioactivities. Five constituents, gallic acid, rutin, ellagic 
      acid, rubusoside, and steviol monoside, were quantified and used in developing 
      qualitative chromatographic fingerprints. The limits of detection and 
      quantification ranged from 0.29 to 37.86 mug/mL. The relative standard deviations 
      (RSDs) of intra- and interday precisions were no more than 3.14 and 3.01%, 
      respectively. The average recoveries were between 93.1 and 97.5%. The developed 
      method was validated in the analysis 14 leaf samples with satisfactory results. 
      The contents of the five marker compounds accounted for an average of about 6% 
      w/w with a variability of 16% among the 14 samples collected from a single site 
      and year. Gallic acid was the least, whereas steviol monoside the most, variable 
      compound among the 14 leaf samples. The characteristic compound rubusoside that 
      is responsible for the sweet taste accounted for 5% of leaf weight. The validated 
      method can now be used to quantitatively and qualitatively assess the quality of 
      R. suavissimus leaves as traditional beverage or potential medicines.
FAU - Chou, Guixin
AU  - Chou G
AD  - School of Renewable Natural Resources, Louisiana State University Agricultural 
      Center, Baton Rouge, Louisiana 70803, USA.
FAU - Xu, Shun-Jun
AU  - Xu SJ
FAU - Liu, Dong
AU  - Liu D
FAU - Koh, Gar Yee
AU  - Koh GY
FAU - Zhang, Jian
AU  - Zhang J
FAU - Liu, Zhijun
AU  - Liu Z
LA  - eng
GR  - R21 AT002882/AT/NCCIH NIH HHS/United States
GR  - R21 AT002882-01A2/AT/NCCIH NIH HHS/United States
GR  - R21AT002882/AT/NCCIH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Diterpenes, Kaurane)
RN  - 0 (Glucosides)
RN  - 19YRN3ZS9P (Ellagic Acid)
RN  - 5G06TVY3R7 (Rutin)
RN  - 60129-60-4 (glucosilsteviol)
RN  - 632XD903SP (Gallic Acid)
RN  - TCV5K3M3GX (rubusoside)
SB  - IM
MH  - Chromatography, High Pressure Liquid/methods
MH  - Diterpenes, Kaurane/analysis
MH  - Ellagic Acid/analysis
MH  - Gallic Acid/analysis
MH  - Glucosides/analysis
MH  - Plant Leaves/*chemistry
MH  - Reproducibility of Results
MH  - Rosaceae/*chemistry
MH  - Rutin/analysis
MH  - Sensitivity and Specificity
PMC - PMC2680688
MID - NIHMS87845
EDAT- 2009/01/14 09:00
MHDA- 2009/03/03 09:00
PMCR- 2010/02/11
CRDT- 2009/01/14 09:00
PHST- 2009/01/14 09:00 [entrez]
PHST- 2009/01/14 09:00 [pubmed]
PHST- 2009/03/03 09:00 [medline]
PHST- 2010/02/11 00:00 [pmc-release]
AID - 10.1021/jf8029397 [pii]
AID - 10.1021/jf8029397 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2009 Feb 11;57(3):1076-83. doi: 10.1021/jf8029397.