PMID- 19140086
OWN - NLM
STAT- MEDLINE
DCOM- 20090707
LR  - 20191210
IS  - 1502-7686 (Electronic)
IS  - 0036-5513 (Linking)
VI  - 69
IP  - 3
DP  - 2009
TI  - A six-colour flow cytometric method for simultaneous detection of cell phenotype 
      and apoptosis of circulating endothelial cells.
PG  - 433-8
LID - 10.1080/00365510802673175 [doi]
AB  - Blood circulating endothelial cells (CECs), with their resting and activated 
      subsets, (rCECs and aCECs) and circulating progenitors cells (CEPs) are two 
      extremely rare cell populations that are important in tissue vascularization. 
      Their number and function are modulated in diseases involving vascular injury, 
      such as human tumours. Although a consensus on the phenotypic definition of 
      endothelial cells, as well as on the optimal enumeration technique, is still 
      lacking, the number of clinical studies based on assessment of these cells is 
      rapidly expanding, as well as the analytical methods employed. The present study 
      aimed to develop a rapid and sensitive flow cytometric method of quantifying and 
      characterizing CECs (with both their subsets and the apoptotic fraction) and 
      CEPs. We analysed peripheral blood samples from 21 subjects with a six-colour 
      flow cytometric approach allowing detection of the cell phenotype of CECs and 
      CEPs using a monoclonal antibodies panel and a dedicated gating strategy. 
      Apoptotic CECs were detected with Annexin V and dead cells with 
      7-amino-actinomycin D staining. The described technique proved to be a new, 
      reliable, tool increasing our knowledge of the biology of CECs and CEPs and can 
      readily be applied in the study of many pathological conditions characterized by 
      endothelial damage.
FAU - Mariucci, S
AU  - Mariucci S
AD  - Flow Cytometry and Cellular Therapy Unit, Medical Oncology, IRCCS Foundation San 
      Matteo, Pavia, Italy. mariuccisara@hotmail.com
FAU - Rovati, B
AU  - Rovati B
FAU - Chatzileontiadou, S
AU  - Chatzileontiadou S
FAU - Bencardino, K
AU  - Bencardino K
FAU - Manzoni, M
AU  - Manzoni M
FAU - Delfanti, S
AU  - Delfanti S
FAU - Danova, M
AU  - Danova M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Clin Lab Invest
JT  - Scandinavian journal of clinical and laboratory investigation
JID - 0404375
RN  - 0 (Annexin A5)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 7240-37-1 (7-aminoactinomycin D)
SB  - IM
MH  - Adult
MH  - Annexin A5
MH  - *Apoptosis
MH  - Blood Cells/*cytology
MH  - Color
MH  - Dactinomycin/analogs & derivatives
MH  - Endothelial Cells/*cytology
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Phenotype
MH  - Sensitivity and Specificity
EDAT- 2009/01/14 09:00
MHDA- 2009/07/08 09:00
CRDT- 2009/01/14 09:00
PHST- 2009/01/14 09:00 [entrez]
PHST- 2009/01/14 09:00 [pubmed]
PHST- 2009/07/08 09:00 [medline]
AID - 907738587 [pii]
AID - 10.1080/00365510802673175 [doi]
PST - ppublish
SO  - Scand J Clin Lab Invest. 2009;69(3):433-8. doi: 10.1080/00365510802673175.