PMID- 19140137
OWN - NLM
STAT- MEDLINE
DCOM- 20090316
LR  - 20131121
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 219
IP  - 2
DP  - 2009 May
TI  - Cell density-dependent changes in intracellular Ca2+ mobilization via the P2Y2 
      receptor in rat bone marrow stromal cells.
PG  - 372-81
LID - 10.1002/jcp.21680 [doi]
AB  - Bone marrow stromal cells (BMSCs) are an interesting subject of research because 
      they have characteristics of mesenchymal stem cells. We investigated 
      intracellular Ca(2+) signaling in rat BMSCs. Agonists for purinergic receptors 
      increased intracellular Ca(2+) levels ([Ca(2+)](i)). The order of potency 
      followed ATP = UTP > ADP = UDP. ATP-induced rise in [Ca(2+)](i) was suppressed by 
      U73122 and suramin, but not by pyridoxalphosphate-6-azophenyl-2',4'-disulfonic 
      acid (PPADS), suggesting the functional expression of G protein-coupled P2Y(2) 
      receptors. RT-PCR and immunohistochemical studies also showed the expression of 
      P2Y(2) receptors. [Ca(2+)](i) response to UTP changed with cell density. The 
      UTP-induced rise in [Ca(2+)](i) was greatest at high density. V(max) (maximum 
      Ca(2+) response) and EC(50) (agonist concentration that evokes 50% of V(max)) 
      suggest that the amount and property of P2Y(2) receptors were changed by cell 
      density. Note that UTP induced Ca(2+) oscillation at only medium cell density. 
      Pharmacological studies indicated that UTP-induced Ca(2+) oscillation required 
      Ca(2+) influx by store-operated Ca(2+) entry. Carbenoxolone, a gap junction 
      blocker, enhanced Ca(2+) oscillation. Immunohistochemical and quantitative 
      real-time PCR studies revealed that proliferating cell nuclear antigen 
      (PCNA)-positive cells declined but the mRNA expression level of the P2Y(2) 
      receptor increased as cell density increased. Co-application of fetal calf serum 
      with UTP induced Ca(2+) oscillation at high cell density. These results suggest 
      that the different patterns observed for [Ca(2+)](i) mobilization with respect to 
      cell density may be associated with cell cycle progression.
FAU - Ichikawa, Jun
AU  - Ichikawa J
AD  - Department of Physiology 2, Kansai Medical University, Osaka, Japan. 
      ichikawj@takii.kmu.ac.jp
FAU - Gemba, Hisae
AU  - Gemba H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels)
RN  - 0 (P2RY2 protein, human)
RN  - 0 (P2ry2 protein, rat)
RN  - 0 (Purinergic P2 Receptor Agonists)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Receptors, Purinergic P2Y2)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - MM6384NG73 (Carbenoxolone)
RN  - SY7Q814VUP (Calcium)
RN  - UT0S826Z60 (Uridine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Bone Marrow Cells/cytology/*physiology
MH  - Calcium/*metabolism
MH  - Calcium Channel Blockers/metabolism
MH  - Calcium Channels/metabolism
MH  - Calcium Signaling/*physiology
MH  - Carbenoxolone/metabolism
MH  - *Cell Count
MH  - Humans
MH  - Male
MH  - Purinergic P2 Receptor Agonists
MH  - Rats
MH  - Rats, Inbred F344
MH  - Receptors, Purinergic P2/genetics/*metabolism
MH  - Receptors, Purinergic P2Y2
MH  - Stromal Cells/cytology/*physiology
MH  - Uridine Triphosphate/metabolism
EDAT- 2009/01/14 09:00
MHDA- 2009/03/17 09:00
CRDT- 2009/01/14 09:00
PHST- 2009/01/14 09:00 [entrez]
PHST- 2009/01/14 09:00 [pubmed]
PHST- 2009/03/17 09:00 [medline]
AID - 10.1002/jcp.21680 [doi]
PST - ppublish
SO  - J Cell Physiol. 2009 May;219(2):372-81. doi: 10.1002/jcp.21680.