PMID- 19140435
OWN - NLM
STAT- MEDLINE
DCOM- 20090227
LR  - 20090114
IS  - 0564-3783 (Print)
IS  - 0564-3783 (Linking)
VI  - 42
IP  - 5
DP  - 2008 Sep-Oct
TI  - Fluorescence intensity profiles of in situ hybridization signals depict genome 
      architecture within human interphase nuclei.
PG  - 3-8
AB  - An approach towards construction of two-dimensional (2D) and three-dimensional 
      (3D) profiles of interphase chromatin architecture by quantification of 
      fluorescence in situ hybridization (FISH) signal intensity is proposed. The 
      technique was applied for analysis of signal intensity and distribution within 
      interphase nuclei of somatic cells in different human tissues. Whole genomic DNA, 
      fraction of repeated DNA sequences (Cot 1) and cloned satellite DNA were used as 
      probes for FISH. The 2D and 3D fluorescence intensity profiles were able to 
      depict FISH signal associations and somatic chromosome pairing. Furthermore, it 
      allowed the detection of replicating signal patterns, the assessment of 
      hybridization efficiency, and comparative analysis of DNA content variation of 
      specific heterochromatic chromosomal regions. The 3D fluorescence intensity 
      profiles allowed the analysis of intensity gradient within the signal volume. An 
      approach was found applicable for determination of assembly of different types of 
      DNA sequences, including classical satellite and alphoid DNA, gene-rich 
      (G-negative bands) and gene-poor (G-positive bands) chromosomal regions as well 
      as for assessment of chromatin architecture and targeted DNA sequence 
      distribution within interphase nuclei. We conclude the approach to be a powerful 
      additional tool for analysis of interphase genome architecture and chromosome 
      behavior in the nucleus of human somatic cells.
FAU - Iourov, I Y
AU  - Iourov IY
AD  - National Research Center of Mental Health, Russian Academy of Medical Sciences, 
      Moscow, Russia.
FAU - Vorsanova, S G
AU  - Vorsanova SG
FAU - Yurov, Y B
AU  - Yurov YB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ukraine
TA  - Tsitol Genet
JT  - TSitologiia i genetika
JID - 0101671
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Brain/cytology
MH  - Cell Nucleus/*genetics/ultrastructure
MH  - Cells, Cultured
MH  - Chorionic Villi/ultrastructure
MH  - Chromosome Aberrations
MH  - Chromosomes, Human/*ultrastructure
MH  - DNA Probes
MH  - Female
MH  - Fluorescent Dyes
MH  - *Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/*genetics
MH  - Lymphocytes/cytology
MH  - Male
MH  - Nucleic Acid Hybridization
EDAT- 2009/01/15 09:00
MHDA- 2009/02/28 09:00
CRDT- 2009/01/15 09:00
PHST- 2009/01/15 09:00 [entrez]
PHST- 2009/01/15 09:00 [pubmed]
PHST- 2009/02/28 09:00 [medline]
PST - ppublish
SO  - Tsitol Genet. 2008 Sep-Oct;42(5):3-8.