PMID- 19141029
OWN - NLM
STAT- MEDLINE
DCOM- 20090601
LR  - 20220330
IS  - 1478-3231 (Electronic)
IS  - 1478-3223 (Linking)
VI  - 29
IP  - 3
DP  - 2009 Mar
TI  - Mesenchymal stem cells from human umbilical cords ameliorate mouse hepatic injury 
      in vivo.
PG  - 356-65
LID - 10.1111/j.1478-3231.2008.01855.x [doi]
AB  - AIMS: To investigate human umbilical cord-derived mesenchymal stem cells 
      (hUCMSCs) for use in the reversal of mouse hepatic injury. METHODS: Human 
      umbilical cord-derived mesenchymal stem cells, characterized by flow cytometry, 
      were transplanted into carbon tetrachloride (CCl(4))-injured mice, and then 
      followed for determination of localization and differentiation. Reverse 
      transcriptase-polymerase chain reaction for the human 17alpha gene and 
      fluorescence in situ hybridization analysis for the human X chromosome were used 
      to locate exogenous hUCMSCs in mouse livers. Peripheral blood and liver specimens 
      were collected at 7, 14 and 21 days after transplantation. For evaluating the 
      recovery of injured liver tissues, serum aminotransferase was measured, and the 
      pathological state of the hepatocytes was assessed. RESULTS: The hUCMSCs were 
      positive for the human MSC-specific markers CD13, CD29, CD44, CD105 and nerve 
      growth factor receptor, but negative for the haematopoietic lineage markers CD31, 
      CD34, CD38, CD45 and HLA-DR. Under conditions favouring differentiation in vivo, 
      the expression of tryptophan 2,3-dioxygenase, human alpha-fetoprotein, 
      cytokeratin 18, fibroblast secretory protein 1 and alpha-smooth-muscle-actin was 
      detectable after hUCMSCs administration to mice subjected to liver injury. 
      Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate 
      (dUTP)-biotin nick end labelling and proliferating cell nuclear antigen staining 
      showed that transplanted hUCMSCs could inhibit hepatocyte apoptosis and 
      facilitate proliferation. Serum aminotransferases were decreased after 
      transplantation of hUCMSCs into the injured mice, and hepatocyte denaturation was 
      reduced. CONCLUSIONS: Human umbilical cord-derived mesenchymal stem cells can 
      enhance recovery of CCl(4)-injured mouse liver, providing evidence that such 
      therapy could be useful for liver disorders or injury.
FAU - Yan, Yongmin
AU  - Yan Y
AD  - School of Medical Technology, Jiangsu University, Jiangsu, China.
FAU - Xu, Wenrong
AU  - Xu W
FAU - Qian, Hui
AU  - Qian H
FAU - Si, Yuan
AU  - Si Y
FAU - Zhu, Wei
AU  - Zhu W
FAU - Cao, Huiling
AU  - Cao H
FAU - Zhou, Hongxing
AU  - Zhou H
FAU - Mao, Fei
AU  - Mao F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081229
PL  - United States
TA  - Liver Int
JT  - Liver international : official journal of the International Association for the 
      Study of the Liver
JID - 101160857
RN  - 0 (DNA Primers)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
RN  - EC 2.6.1.- (Transaminases)
SB  - IM
MH  - Animals
MH  - Carbon Tetrachloride/toxicity
MH  - Cell Differentiation/physiology
MH  - Chemical and Drug Induced Liver Injury
MH  - DNA Primers/genetics
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Liver Diseases/pathology/*therapy
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mice
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transaminases/blood
MH  - Umbilical Cord/*cytology
EDAT- 2009/01/15 09:00
MHDA- 2009/06/02 09:00
CRDT- 2009/01/15 09:00
PHST- 2009/01/15 09:00 [entrez]
PHST- 2009/01/15 09:00 [pubmed]
PHST- 2009/06/02 09:00 [medline]
AID - LIV1855 [pii]
AID - 10.1111/j.1478-3231.2008.01855.x [doi]
PST - ppublish
SO  - Liver Int. 2009 Mar;29(3):356-65. doi: 10.1111/j.1478-3231.2008.01855.x. Epub 
      2008 Dec 29.