PMID- 19141071
OWN - NLM
STAT- MEDLINE
DCOM- 20090422
LR  - 20220317
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 108
IP  - 5
DP  - 2009 Mar
TI  - The CREB/CRE transcriptional pathway: protection against oxidative 
      stress-mediated neuronal cell death.
PG  - 1251-65
LID - 10.1111/j.1471-4159.2008.05864.x [doi]
AB  - Formation of reactive oxygen and nitrogen species is a precipitating event in an 
      array of neuropathological conditions. In response to excessive reactive oxygen 
      species (ROS) levels, transcriptionally dependent mechanisms drive the 
      up-regulation of ROS scavenging proteins which, in turn, limit the extent of 
      brain damage. Here, we employed a transgenic approach in which cAMP-response 
      element binding protein (CREB)-mediated transcription is repressed (via A-CREB) 
      to examine the contribution of the CREB/cAMP response element pathway to 
      neuroprotection and its potential role in limiting ROS toxicity. Using the 
      pilocarpine-evoked repetitive seizure model, we detected a marked enhancement of 
      cell death in A-CREB transgenic mice. Paralleling this, there was a dramatic 
      increase in tyrosine nitration (a marker of reactive species formation) in A-CREB 
      transgenic mice. In addition, inducible expression of peroxisome 
      proliferator-activated receptor gamma coactivator-1alpha was diminished in A-CREB 
      transgenic mice, as was activity of complex I of the mitochondrial electron 
      transport chain. Finally, the neuroprotective effect of brain-derived 
      neurotrophic factor (BDNF) against ROS-mediated cell death was abrogated by 
      disruption of CREB-mediated transcription. Together, these data both extend our 
      understanding of CREB functionality and provide in vivo validation for a model in 
      which CREB functions as a pivotal upstream integrator of neuroprotective 
      signaling against ROS-mediated cell death.
FAU - Lee, Boyoung
AU  - Lee B
AD  - Department of Neuroscience, Ohio State University, Columbus, 43210, USA.
FAU - Cao, Ruifeng
AU  - Cao R
FAU - Choi, Yun-Sik
AU  - Choi YS
FAU - Cho, Hee-Yeon
AU  - Cho HY
FAU - Rhee, Alex D
AU  - Rhee AD
FAU - Hah, Cyrus K
AU  - Hah CK
FAU - Hoyt, Kari R
AU  - Hoyt KR
FAU - Obrietan, Karl
AU  - Obrietan K
LA  - eng
GR  - R01 MH062335-05/MH/NIMH NIH HHS/United States
GR  - NS47176/NS/NINDS NIH HHS/United States
GR  - MH62335/MH/NIMH NIH HHS/United States
GR  - R01 NS047176-04/NS/NINDS NIH HHS/United States
GR  - R01 MH062335-07/MH/NIMH NIH HHS/United States
GR  - R01 MH062335/MH/NIMH NIH HHS/United States
GR  - R01 NS047176/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090128
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (A-CREB protein)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Fluoresceins)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Organic Chemicals)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Ppargc1a protein, mouse)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (fluoro jade)
RN  - 01MI4Q9DI3 (Pilocarpine)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 7C0697DR9I (Atropine)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 5.2.1.- (Cyclophilins)
SB  - IM
MH  - Animals
MH  - Atropine
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cells, Cultured
MH  - Corpus Striatum/cytology
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Cyclophilins/genetics/metabolism
MH  - Disease Models, Animal
MH  - Electroencephalography
MH  - Embryo, Mammalian
MH  - Fluoresceins
MH  - Green Fluorescent Proteins/genetics
MH  - Heme Oxygenase-1/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Multienzyme Complexes/metabolism
MH  - Neurons/drug effects/*physiology
MH  - Organic Chemicals/metabolism
MH  - Oxidative Stress/drug effects/*physiology
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - Pilocarpine
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Signal Transduction/*physiology
MH  - Status Epilepticus/chemically induced/*pathology
MH  - Trans-Activators/genetics/metabolism
MH  - Transcription Factors
MH  - Transfection/methods
PMC - PMC2884273
MID - NIHMS105454
EDAT- 2009/01/15 09:00
MHDA- 2009/04/23 09:00
PMCR- 2010/06/14
CRDT- 2009/01/15 09:00
PHST- 2009/01/15 09:00 [entrez]
PHST- 2009/01/15 09:00 [pubmed]
PHST- 2009/04/23 09:00 [medline]
PHST- 2010/06/14 00:00 [pmc-release]
AID - JNC5864 [pii]
AID - 10.1111/j.1471-4159.2008.05864.x [doi]
PST - ppublish
SO  - J Neurochem. 2009 Mar;108(5):1251-65. doi: 10.1111/j.1471-4159.2008.05864.x. Epub 
      2009 Jan 28.