PMID- 19141193
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20211203
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 21
IP  - 1
DP  - 2009 Jan
TI  - [Study of the role of endoplasmic reticulum stress mediated apoptosis signal 
      pathway in sepsis-induced splenic lymphocyte apoptosis].
PG  - 48-50
AB  - OBJECTIVE: To investigate the role of endoplasmic reticulum (ER) stress-mediated 
      apoptosis signal pathway in extensive apoptosis of splenic lymphocytes in sepsis. 
      METHODS: Twenty-four C57BL/6 mice were randomized into the cecal ligation and 
      puncture (CLP) group and sham operation group, with 12 mice in each group. 
      Twenty-four hours after CLP, the animals were sacrificed, and spleens were 
      harvested for evaluation. Apoptosis was detected by the terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) analysis. The expressions of 
      glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) were 
      detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western 
      blotting. RESULTS: High degree of lymphocyte apoptosis was observed in the CLP 
      group. Meanwhile, marked induction of GRP78 expression was observed in the 
      splenocytes in septic mice, indicating the motivation of the unfolded protein 
      responses (UPR). Furthermore, both mRNA and protein levels of CHOP were highly 
      upregulated in the CLP group, suggesting that ER stress response was switched to 
      a pro-apoptotic response. CONCLUSION: These results demonstrates activation of 
      the ER stress response in lymphocytes and that ER stress may contribute to 
      abnormal lymphocyte apoptosis during sepsis.
FAU - Ma, Tao
AU  - Ma T
AD  - Department of Surgery, General Hospital, Tianjin Medical University, Tianjin 
      300052, China.
FAU - Han, Lu
AU  - Han L
FAU - Hu, Wen-quan
AU  - Hu WQ
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Hspa5 protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (glucose-regulated proteins)
RN  - 147336-12-7 (Transcription Factor CHOP)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Disease Models, Animal
MH  - Endoplasmic Reticulum/*metabolism
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - HSP70 Heat-Shock Proteins/genetics/metabolism
MH  - Lymphocytes/metabolism/*pathology
MH  - Membrane Proteins/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/genetics
MH  - Random Allocation
MH  - Sepsis/*metabolism/pathology
MH  - *Signal Transduction
MH  - Spleen/metabolism/*pathology
MH  - Transcription Factor CHOP/genetics/metabolism
EDAT- 2009/01/15 09:00
MHDA- 2010/05/07 06:00
CRDT- 2009/01/15 09:00
PHST- 2009/01/15 09:00 [entrez]
PHST- 2009/01/15 09:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009 Jan;21(1):48-50.