PMID- 19141474
OWN - NLM
STAT- MEDLINE
DCOM- 20090203
LR  - 20220309
IS  - 0890-9369 (Print)
IS  - 1549-5477 (Electronic)
IS  - 0890-9369 (Linking)
VI  - 22
IP  - 24
DP  - 2008 Dec 15
TI  - The A- and B-type nuclear lamin networks: microdomains involved in chromatin 
      organization and transcription.
PG  - 3409-21
LID - 10.1101/gad.1735208 [doi]
AB  - The nuclear lamins function in the regulation of replication, transcription, and 
      epigenetic modifications of chromatin. However, the mechanisms responsible for 
      these lamin functions are poorly understood. We demonstrate that A- and B-type 
      lamins form separate, but interacting, stable meshworks in the lamina and have 
      different mobilities in the nucleoplasm as determined by fluorescence correlation 
      spectroscopy (FCS). Silencing lamin B1 (LB1) expression dramatically increases 
      the lamina meshwork size and the mobility of nucleoplasmic lamin A (LA). The 
      changes in lamina mesh size are coupled to the formation of LA/C-rich nuclear 
      envelope blebs deficient in LB2. Comparative genomic hybridization (CGH) analyses 
      of microdissected blebs, fluorescence in situ hybridization (FISH), and 
      immunofluorescence localization of modified histones demonstrate that gene-rich 
      euchromatin associates with the LA/C blebs. Enrichment of hyperphosphorylated RNA 
      polymerase II (Pol II) and histone marks for active transcription suggest that 
      blebs are transcriptionally active. However, in vivo labeling of RNA indicates 
      that transcription is decreased, suggesting that the LA/C-rich microenvironment 
      induces promoter proximal stalling of Pol II. We propose that different lamins 
      are organized into separate, but interacting, microdomains and that LB1 is 
      essential for their organization. Our evidence suggests that the organization and 
      regulation of chromatin are influenced by interconnections between these lamin 
      microdomains.
FAU - Shimi, Takeshi
AU  - Shimi T
AD  - Department of Cell and Molecular Biology, Northwestern University, Feinberg 
      School of Medicine, Chicago, Illinois 60611, USA.
FAU - Pfleghaar, Katrin
AU  - Pfleghaar K
FAU - Kojima, Shin-ichiro
AU  - Kojima S
FAU - Pack, Chan-Gi
AU  - Pack CG
FAU - Solovei, Irina
AU  - Solovei I
FAU - Goldman, Anne E
AU  - Goldman AE
FAU - Adam, Stephen A
AU  - Adam SA
FAU - Shumaker, Dale K
AU  - Shumaker DK
FAU - Kinjo, Masataka
AU  - Kinjo M
FAU - Cremer, Thomas
AU  - Cremer T
FAU - Goldman, Robert D
AU  - Goldman RD
LA  - eng
GR  - R01 AG023776/AG/NIA NIH HHS/United States
GR  - R01 CA031760/CA/NCI NIH HHS/United States
GR  - R01CA31760/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (CKAP2 protein, human)
RN  - 0 (Chromatin)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (LMNA protein, human)
RN  - 0 (Lamin Type A)
RN  - EC 2.7.7.7 (DNA Polymerase II)
SB  - IM
CIN - Nat Rev Mol Cell Biol. 2012 Mar;13(3):140. PMID: 22334141
MH  - Chromatin/*genetics/*metabolism
MH  - Cytoskeletal Proteins/*genetics
MH  - DNA Polymerase II/metabolism
MH  - *Gene Expression Regulation
MH  - Gene Silencing
MH  - HeLa Cells
MH  - Humans
MH  - Lamin Type A/*genetics
MH  - Nuclear Lamina/metabolism
PMC - PMC2607069
EDAT- 2009/01/15 09:00
MHDA- 2009/02/04 09:00
PMCR- 2009/06/15
CRDT- 2009/01/15 09:00
PHST- 2009/01/15 09:00 [entrez]
PHST- 2009/01/15 09:00 [pubmed]
PHST- 2009/02/04 09:00 [medline]
PHST- 2009/06/15 00:00 [pmc-release]
AID - 22/24/3409 [pii]
AID - 10.1101/gad.1735208 [doi]
PST - ppublish
SO  - Genes Dev. 2008 Dec 15;22(24):3409-21. doi: 10.1101/gad.1735208.