PMID- 19141974
OWN - NLM
STAT- MEDLINE
DCOM- 20110401
LR  - 20130527
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 33
IP  - 12
DP  - 2008 Dec
TI  - Protective effects of alanyl-glutamine on acute lung injury induced by 
      lipopolysaccharide in rats.
PG  - 1095-100
AB  - OBJECTIVE: To investigate the effect of alanyl-glutamine (Ala-Gln) on acute lung 
      injury (ALI) in rats induced by sepsis and its mechanisms. METHODS: Forty healthy 
      adult Wistar rats were randomly divided into a control group, a 
      lipopolysaccharide (LPS)-induced shock group, an Ala-Gln treated group, and a 
      glutamine (Gln) treated group. The control group received an intravenous infusion 
      of 28 mL/kg lactated Ringeros solution(LR). The LPS-induced shock group received 
      an intravenous administration of 25 mL/kg LR, and then 3 mL/kg (6 mg/kg) LPS 
      (L-2880, Sigma, America). The Ala-Gln treated group received 4.5% Dipeptiven (25 
      mL/kg, equaling 0.75 g/kg Gln) immediately before 3 mL/kg (6 mg/kg) LPS.The Gln 
      treated group received 3% glutamine ( 25 mL/kg, 0.75 g/kg) immediately before 3 
      mL/kg (6 mg/kg) LPS. Serum (1 mL) was drawn via the femoral vein or cardiac 
      puncture before LPS injection (T(0)) and 6 h after the administration of LPS 
      (T(1)), respectively. All rats were killed 6 h after LPS infusion. The samples of 
      pulmonary tissue and lung lavage fluid were collected after experiments. Tumor 
      necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and 
      interleukin-8 (IL-8) in the serum at T(0) and T(1) were detected by ELISA. 
      Apoptosis in the lung epithelial cells was detected with TUNEL assays. The lung 
      wet/dry(W/D)weight ratio and total protein in the bronchoalveolar lavage fluid 
      (BALF) were measured. RESULTS: Six hours after the infusion of LPS (T(1)), the 
      plasma concentrations of TNF-alpha, IL-1beta, and IL-8 were much lower in the 
      Ala-Gln treated group and the Gln treated group than those in the LPS-induced 
      shock group (P<0.05). Compared with the LPS-induced shock group, Ala-Gln and Gln 
      significantly reduced the increase in the lung wet/dry weight ratio (P<0.05) and 
      attenuated the morphological lung damage. CONCLUSION: Intravenous administration 
      of Ala-Gln can effectively protect the lung from sepsis induced injury.
FAU - Liu, Jing-Chen
AU  - Liu JC
AD  - Department of Anesthesiology, First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530021, China. liujcnn@163.com
FAU - Wang, Hai-Tang
AU  - Wang HT
FAU - Wang, Wei
AU  - Wang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Dipeptides)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Protective Agents)
RN  - U5JDO2770Z (alanylglutamine)
SB  - IM
MH  - Animals
MH  - Dipeptides/pharmacology/*therapeutic use
MH  - Lipopolysaccharides
MH  - Lung Injury/*drug therapy/etiology
MH  - Protective Agents/pharmacology/*therapeutic use
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Shock, Septic/*chemically induced/complications
EDAT- 2009/01/15 09:00
MHDA- 2011/04/02 06:00
CRDT- 2009/01/15 09:00
PHST- 2009/01/15 09:00 [entrez]
PHST- 2009/01/15 09:00 [pubmed]
PHST- 2011/04/02 06:00 [medline]
AID - 1672-7347(2008)12-1095-06 [pii]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Dec;33(12):1095-100.