PMID- 1914258
OWN - NLM
STAT- MEDLINE
DCOM- 19911121
LR  - 20190820
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 61
IP  - 2 Pt 1
DP  - 1991 Nov
TI  - Infection of "nonprofessional phagocytes" with Mycobacterium avium complex.
PG  - 225-35
AB  - Organisms belonging to the Mycobacterium avium complex are the most common 
      bacteria isolated from patients with AIDS. In these patients, M. avium is 
      associated with disseminated disease, and bacteria are found within macrophages 
      in the liver and spleen. To examine the potential of M. avium infection of a 
      nonprofessional phagocyte, the murine fibroblast cell line (L929 cells) are 
      infected with strain 101 (serotype 1) of M. avium. The duplication time for the 
      intracellular bacteria was approximately 36 hr. Progressive intracellular growth 
      ultimately resulted in the destruction of infected cells (after approximately 12 
      days in culture). Supernatant obtained from infected L929 cells contained 
      interferon-beta (IFN beta) and granulocyte macrophage colony stimulating factor 
      (GM-CSF) (50 +/- 12 ng/10(5) cells and 63 +/- 18 pg/10(5) cells, respectively, by 
      18 hr). IFN beta could be detected by 3 hr after infection, while GM-CSF was 
      first detected by 6 hr. Release of IFN beta by infected L929 cells could 
      subsequently stimulate NK cells, but not macrophages, to lyse infected L929 
      cells. These data using L929 cells suggest that M. avium may invade fibroblasts 
      during the course of the infection and that fibroblast infection may trigger NK 
      cell-mediated cytotoxicity against the infected fibroblasts.
FAU - Bermudez, L E
AU  - Bermudez LE
AD  - Kuzell Institute for Arthritis and Infectious Diseases, Pacific Presbyterian 
      Medical Center, San Francisco, California 94115.
LA  - eng
GR  - 5 RO1 AI 25769-03/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cytokines/analysis/pharmacology
MH  - Cytotoxicity, Immunologic/drug effects
MH  - Fibroblasts/microbiology
MH  - Humans
MH  - Killer Cells, Natural/immunology
MH  - Macrophages/immunology
MH  - Mice
MH  - Mycobacterium avium Complex/drug effects/*growth & development
MH  - Phagocytes/*microbiology
EDAT- 1991/11/01 00:00
MHDA- 1991/11/01 00:01
CRDT- 1991/11/01 00:00
PHST- 1991/11/01 00:00 [pubmed]
PHST- 1991/11/01 00:01 [medline]
PHST- 1991/11/01 00:00 [entrez]
AID - 10.1016/s0090-1229(05)80026-1 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1991 Nov;61(2 Pt 1):225-35. doi: 
      10.1016/s0090-1229(05)80026-1.