PMID- 19142676
OWN - NLM
STAT- MEDLINE
DCOM- 20090504
LR  - 20211020
IS  - 1439-1104 (Electronic)
IS  - 1354-2516 (Linking)
VI  - 9
IP  - 1
DP  - 2009 Mar
TI  - Molecular identification and expression of the NMDA receptor NR1 subunit in the 
      leech.
PG  - 11-20
LID - 10.1007/s10158-008-0085-3 [doi]
AB  - The N-methyl-D-aspartate receptor (NMDAR) is involved in a number of 
      physiological and pathophysiological processes in vertebrates, but there have 
      been few studies examining the role of invertebrate NMDA receptors. In the leech, 
      pharmacological evidence suggests that NMDARs contribute to synaptic plasticity, 
      but there has been no molecular identification of NMDA receptors. In this report, 
      a partial cDNA encoding the leech NR1 subunit of the NMDA receptor (HirNR1) is 
      presented. Reverse transcriptase-polymerase chain reaction from single neurons of 
      the leech central nervous system confirms HirNR1 expression in the Retzius (R), 
      Anterior Pagoda (AP), Pressure (P), and Touch (T) neurons. Immunoblotting with an 
      anti-NR1 antibody yielded a approximately 110 kDa protein, similar to the 
      expected weight of the NR1 subunit (approximately 116 kDa). Finally, pairing pre- 
      and postsynaptic activity elicited long-term potentiation in synapses between 
      neurons expressing NR1 mRNA (P-to-AP synapse) and this potentiation was blocked 
      by the NMDAR antagonist AP5.
FAU - Grey, Kathryn B
AU  - Grey KB
AD  - Division of Basic Biomedical Sciences, Neuroscience Group, Sanford School of 
      Medicine, University of South Dakota, Vermillion, SD 57069, USA.
FAU - Moss, Brenda L
AU  - Moss BL
FAU - Burrell, Brian D
AU  - Burrell BD
LA  - eng
GR  - P20 RR015567/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090114
PL  - Germany
TA  - Invert Neurosci
JT  - Invertebrate neuroscience : IN
JID - 9602489
RN  - 0 (DNA, Complementary)
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - Central Nervous System/metabolism
MH  - DNA, Complementary
MH  - Databases, Genetic
MH  - Excitatory Postsynaptic Potentials
MH  - Gene Expression
MH  - Leeches/genetics/*metabolism
MH  - Long-Term Potentiation
MH  - Microelectrodes
MH  - Molecular Sequence Data
MH  - Neurons/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & 
      inhibitors/chemistry/*genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Synaptic Transmission
EDAT- 2009/01/15 09:00
MHDA- 2009/05/05 09:00
CRDT- 2009/01/15 09:00
PHST- 2008/10/24 00:00 [received]
PHST- 2008/12/17 00:00 [accepted]
PHST- 2009/01/15 09:00 [entrez]
PHST- 2009/01/15 09:00 [pubmed]
PHST- 2009/05/05 09:00 [medline]
AID - 10.1007/s10158-008-0085-3 [doi]
PST - ppublish
SO  - Invert Neurosci. 2009 Mar;9(1):11-20. doi: 10.1007/s10158-008-0085-3. Epub 2009 
      Jan 14.